Re-epithelialization and immune cell behaviour in an ex vivo human skin model.
Reads0
Chats0
TLDR
A novel wound model based on application of negative pressure and its effects for epidermal regeneration and immune cell behaviour is presented, which recapitulates the main features of epithelial wound regeneration, and can be applied for testing wound healing therapies and investigating underlying mechanisms.Abstract:
A large body of literature is available on wound healing in humans. Nonetheless, a standardized ex vivo wound model without disruption of the dermal compartment has not been put forward with compelling justification. Here, we present a novel wound model based on application of negative pressure and its effects for epidermal regeneration and immune cell behaviour. Importantly, the basement membrane remained intact after blister roof removal and keratinocytes were absent in the wounded area. Upon six days of culture, the wound was covered with one to three-cell thick K14+Ki67+ keratinocyte layers, indicating that proliferation and migration were involved in wound closure. After eight to twelve days, a multi-layered epidermis was formed expressing epidermal differentiation markers (K10, filaggrin, DSG-1, CDSN). Investigations about immune cell-specific manners revealed more T cells in the blister roof epidermis compared to normal epidermis. We identified several cell populations in blister roof epidermis and suction blister fluid that are absent in normal epidermis which correlated with their decrease in the dermis, indicating a dermal efflux upon negative pressure. Together, our model recapitulates the main features of epithelial wound regeneration, and can be applied for testing wound healing therapies and investigating underlying mechanisms.read more
Citations
More filters
Journal ArticleDOI
Atmospheric transport is a major pathway of microplastics to remote regions
Nikolaos Evangeliou,Henrik Grythe,Zbigniew Klimont,Chris Heyes,Sabine Eckhardt,Susana Lopez-Aparicio,Andreas Stohl,Andreas Stohl +7 more
TL;DR: It is shown that atmospheric transport is a major pathway for road plastic pollution over remote regions, and it is suggested that the Arctic may be a particularly sensitive receptor region, where the light-absorbing properties of TWPs and BWPs may also cause accelerated warming and melting of the cryosphere.
Journal ArticleDOI
DeepEMhancer: a deep learning solution for cryo-EM volume post-processing.
Rubén J. Sánchez-García,Rubén J. Sánchez-García,Josué Gómez-Blanco,Josué Gómez-Blanco,Ana Maria Cuervo,José María Carazo,Carlos Oscar S. Sorzano,Javier Vargas,Javier Vargas +8 more
TL;DR: DeepEMhancer as mentioned in this paper is a deep learning approach designed to perform automatic post-processing of cryo-EM maps, trained on a dataset of pairs of experimental maps and maps sharpened using their respective atomic models, has learned how to post-process experimental maps performing masking-like and sharpening-like operations.
Journal ArticleDOI
Noisy intermediate-scale quantum algorithms
TL;DR: In this article , the authors discuss what is possible in this ''noisy intermediate scale'' quantum (NISQ) era, including simulation of many-body physics and chemistry, combinatorial optimization, and machine learning.
Journal ArticleDOI
metaFlye: scalable long-read metagenome assembly using repeat graphs
Mikhail Kolmogorov,Derek M. Bickhart,Bahar Behsaz,Alexey Gurevich,Mikhail Rayko,Sung Bong Shin,Kristen L. Kuhn,Jeffrey Yuan,Evgeny Polevikov,Timothy P. L. Smith,Pavel A. Pevzner +10 more
TL;DR: MetaFlye is presented, which addresses important long-read metagenomic assembly challenges, such as uneven bacterial composition and intra-species heterogeneity, and benchmarked metaFlye using simulated and mock bacterial communities and show that it consistently produces assemblies with better completeness and contiguity than state-of-the-art long- read assemblers.
Journal ArticleDOI
Recent advances on the removal of dyes from wastewater using various adsorbents: A critical review
TL;DR: In this paper, a comprehensive review of the various types of adsorbents such as commercial activated carbon, metal oxide-based, carbon based, metal-organic framework, and polymer-based adhesives used in dye remediation of contaminated water is presented.
References
More filters
Journal ArticleDOI
Wound repair and regeneration: Mechanisms, signaling, and translation
TL;DR: In this review, emerging concepts in tissue regeneration and repair are highlighted, and some perspectives on how to translate current knowledge into viable clinical approaches for treating patients with wound-healing pathologies are provided.
Journal ArticleDOI
A Structural Scaffolding of Intermediate Filaments in Health and Disease
Elaine Fuchs,Don W. Cleveland +1 more
TL;DR: Evidence is reviewed here that intermediate filaments provide a flexible intracellular scaffolding whose function is to structure cytoplasm and to resist stresses externally applied to the cell.
Journal ArticleDOI
Epithelialization in Wound Healing: A Comprehensive Review
Irena Pastar,Olivera Stojadinovic,Natalie C. Yin,Horacio Ramirez,Aron G. Nusbaum,Andrew P. Sawaya,Shailee Patel,Laiqua Khalid,R. Isseroff,Marjana Tomic-Canic +9 more
TL;DR: The pivotal role of keratinocytes in epithelialization is focused on, including cellular processes and mechanisms of their regulation during re-epithelialization, and their cross talk with other cell types participating in wound healing.
Journal ArticleDOI
Filaggrin in the frontline: role in skin barrier function and disease.
TL;DR: Recent human genetic studies strongly suggest that perturbation of skin barrier function as a result of reduction or complete loss of filaggrin expression leads to enhanced percutaneous transfer of allergens.
Journal ArticleDOI
The Vast Majority of CLA+ T Cells Are Resident in Normal Skin
Rachael A. Clark,Benjamin F. Chong,Nina Mirchandani,Nooshin K. Brinster,Keiichi Yamanaka,Rebecca Dowgiert,Thomas S. Kupper +6 more
TL;DR: There are T cells within normal, noninflamed skin that most likely conduct immunosurveillance and are implicated in the development of psoriasis and it is estimated that 98% of CLA+ effector memory T cells are resident in normal skin under resting conditions.
Related Papers (5)
Occurrence of the potent mutagens 2- nitrobenzanthrone and 3-nitrobenzanthrone in fine airborne particles
In situ immune response and mechanisms of cell damage in central nervous system of fatal cases microcephaly by Zika virus
Raimunda do Socorro da Silva Azevedo,Jorge Rodrigues de Sousa,Marialva Tereza Ferreira de Araújo,Arnaldo Jorge Martins Filho,Bianca Nascimento de Alcantara,Fernanda Montenegro de Carvalho Araújo,Maria G. L. Queiroz,Ana Cecília Ribeiro Cruz,Beatriz H. Baldez Vasconcelos,Jannifer Oliveira Chiang,Lívia Carício Martins,Livia Medeiros Neves Casseb,Eliana Vieira Pinto da Silva,Valéria Lima Carvalho,Barbara Cristina Baldez Vasconcelos,Sueli Rodrigues,Consuelo Silva de Oliveira,Juarez Antônio Simões Quaresma,Pedro Fernando da Costa Vasconcelos +18 more