Recovery and characterization of a minute virus of canines.
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TLDR
The virus did not produce cytopathic effects in primary canine kidney or thymus cell cultures, or in cell cultures of human, simian, porcine, bovine, feline, and murine origin.Abstract:
Four antigenically related transmissible agents were recovered from canine fecal specimens. The agents produced cytopathic effects in a continuous dog cell line developed in this laboratory. Increased antibody titers were demonstrated in three of the four dogs which provided the isolates. The virus did not produce cytopathic effects in primary canine kidney or thymus cell cultures, or in cell cultures of human, simian, porcine, bovine, feline, and murine origin. The agent is resistant to ether, chloroform, and heat treatment, and the growth of the virus is inhibited by 5-iodo-2-deoxyuridine. After acridine orange staining, green fluorescent intranuclear inclusions are seen in infected cell cultures. By electron microscopy, the virions measure approximately 20 to 21 nm in overall diameter and are present in the nuclei of infected cells. These properties are consistent with membership in the parvovirus or picodnavirus group. The agent hemagglutinates rhesus red blood cells at 5 C and by hemagglutination-inhibition tests could be readily distinguished from H-1, rat virus, and the minute virus of mice. Canine gamma globulin contains high titers of neutralizing antibody and neutralizing antibody was found in a high percentage of military dogs and in beagles of a breeding colony.read more
Citations
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Human Bocavirus: Passenger or Pathogen in Acute Respiratory Tract Infections?
Oliver Schildgen,Andreas Müller,Tobias Allander,Ian M. Mackay,Ian M. Mackay,Sebastian Völz,Bernd Kupfer,Arne Simon +7 more
TL;DR: It is suggested that primary HBoV infection causes respiratory tract symptoms which can be followed by prolonged low-level virus shedding in the respiratory tract, and the ability to detect primary infection through the development of improved diagnostic methods will be of great importance for future studies seeking to assign a role for H BoV in causing respiratory illnesses.
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Detection of Human Bocavirus in Japanese Children with Lower Respiratory Tract Infections
Xiaoming Ma,Rika Endo,Nobuhisa Ishiguro,Takashi Ebihara,Hiroaki Ishiko,Tadashi Ariga,Hideaki Kikuta +6 more
TL;DR: A newly cloned human virus of the genus Bocavirus was detected by PCR from nasopharyngeal swab samples collected from children with lower respiratory tract infections, suggesting HBoV may be one of the causative agents of lower respiratory tracts infections in young children.
Journal ArticleDOI
A real-time PCR assay for rapid detection and quantitation of canine parvovirus type 2 in the feces of dogs.
Nicola Decaro,Gabriella Elia,Vito Martella,Costantina Desario,Marco Campolo,Livia Di Trani,Elvira Tarsitano,Maria Tempesta,Canio Buonavoglia +8 more
TL;DR: A rapid, sensitive and reproducible real-time PCR assay for detecting and quantifying canine parvovirus type 2 (CPV-2) DNA in the feces of dogs with diarrhea was described and demonstrated to be highly specific and sensitive.
Journal ArticleDOI
Clinical and Molecular Epidemiology of Human Bocavirus in Respiratory and Fecal Samples from Children in Hong Kong
Susanna K. P. Lau,Cyril C. Y. Yip,Tak-Lun Que,Rodney A. Lee,Rex Au-Yeung,Boping Zhou,Lok-Yee So,Yu-Lung Lau,Kwok-Hung Chan,Patrick C. Y. Woo,Kwok-Yung Yuen +10 more
TL;DR: Human bocavirus was detected in fecal specimens in children with acute gastroenteritis and a single lineage of HBoV was associated with both respiratory tract and enteric infections.
Journal ArticleDOI
Canine host range and a specific epitope map along with variant sequences in the capsid protein gene of canine parvovirus and related feline, mink, and raccoon parvoviruses.
TL;DR: Relationships between the viruses determined from DNA sequences of the capsid protein genes of 10 virus isolates showed the CPV isolates to be closely related to the other viruses, although comprising a distinct group.
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