Reduced invasion to human epithelial cell lines of Salmonella enterica serovar Typhi carrying S. Typhimurium sopD2.
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The results showed that the presence of sopD2(STM) in S. Typhi significantly modified the bacterial ability to alter cellular permeability and decrease the CFUs recovered after cell invasion of human epithelial cell line.Abstract:
Salmonella enterica serovar Typhi and Typhimurium are closely related serovars. However, S. Typhi, a human-specific pathogen, has 5% of genes as pseudogenes, far more than S. Typhimurium, which only has 1%. One of these pseudogenes corresponds to sopD2, which in S. Typhimurium encodes an effector protein involved in Salmonella-containing vacuole biogenesis in human epithelial cell lines, which is needed for full virulence of the pathogen. We investigated whether S. Typhi trans-complemented with the functional sopD2 gene from S. Typhimurium (sopD2(STM) ) would reduce the invasion of human epithelial cell lines. Our results showed that the presence of sopD2(STM) in S. Typhi significantly modified the bacterial ability to alter cellular permeability and decrease the CFUs recovered after cell invasion of human epithelial cell line. These results add to mounting evidence that pseudogenes contribute to S. Typhi adaptation to humans.read more
Citations
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Pseudogenization of sopA and sopE2 is functionally linked and contributes to virulence of Salmonella enterica serovar Typhi
L.M. Valenzuela,Alejandro A. Hidalgo,Luis Rodríguez,I.M. Urrutia,A.P. Ortega,Nicolás A. Villagra,Daniel Paredes-Sabja,Iván L. Calderón,Fernando Gil,Claudia P. Saavedra,Guido C. Mora,Juan A. Fuentes +11 more
TL;DR: S. Typhi expressing S. Typhimurium sopA and/or sopE2 exhibited a decreased invasion in different epithelial cell lines compared with S. typhi WT, suggesting that functional SopA and SopE2 participate concertedly in the invasion process.
References
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Journal ArticleDOI
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
TL;DR: A simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s), which should be widely useful, especially in genome analysis of E. coli and other bacteria.
Journal ArticleDOI
Complete genome sequence of Salmonella enterica serovar Typhimurium LT2
Michael McClelland,Kenneth E. Sanderson,John Spieth,Sandra W. Clifton,Phil Latreille,Laura Courtney,Steffen Porwollik,Johar Ali,Mike Dante,Feiyu Du,Shunfang Hou,Dan Layman,Shawn Leonard,Christine Nguyen,Kelsi Scott,Andrea Holmes,Neenu Grewal,Elizabeth Mulvaney,Ellen E. Ryan,Hui Sun,Liliana Florea,Liliana Florea,Webb Miller,Tamberlyn Stoneking,Michael Nhan,Robert H. Waterston,Richard K. Wilson +26 more
TL;DR: The distribution of close homologues of S. typhimurium LT2 genes in eight related enterobacteria was determined using previously completed genomes of three related bacteria, sample sequencing of both S. enterica serovar Paratyphi A and Klebsiella pneumoniae as mentioned in this paper.
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Julian Parkhill,Gordon Dougan,Keith D. James,Nicholas R. Thomson,Derek Pickard,John Wain,Carol Churcher,Karen Mungall,Stephen D. Bentley,Matthew T. G. Holden,Mohammed Sebaihia,Stephen Baker,D. Basham,Karen Brooks,Tracey Chillingworth,Phillippa L. Connerton,A. Cronin,P. Davis,Robert L. Davies,L. Dowd,Nicholas J. White,Jeremy Farrar,Theresa Feltwell,N. Hamlin,Ashraful Haque,Tran Tinh Hien,S. Holroyd,Kay Jagels,Anders Krogh,Torben Larsen,S. Leather,Sharon Moule,Peadar O'Gaora,Christopher M. Parry,Michael A. Quail,Kim Rutherford,Mark Simmonds,Jason Skelton,K. Stevens,Sally Whitehead,Bart Barrell +40 more
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Genes Lost and Genes Found: Evolution of Bacterial Pathogenesis and Symbiosis
Howard Ochman,Nancy A. Moran +1 more
TL;DR: This work has shown that changes in genome repertoire, occurring through gene acquisition and deletion, are the major events underlying the emergence and evolution of bacterial pathogens and symbionts.
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Salmonella typhimurium attachment to human intestinal epithelial monolayers: transcellular signalling to subepithelial neutrophils.
TL;DR: A novel transcellular pathway exists in which subepithelial PMN respond to lumenal pathogens across a functionally intact epithelium, and it is speculated that IL-8 may act in concert with an as yet unidentified trans cellular chemotactic factor(s) (TCF) which directs PMN migration across the intestinal epithelia.
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