Regulation of human thymidine kinase during the cell cycle
James L. Sherley,Thomas J. Kelly +1 more
Reads0
Chats0
TLDR
Two different post-transcriptional mechanisms largely account for the periodic behavior of the enzyme activity during the cell cycle, indicating that the efficiency of translation of thymidine kinase mRNA increases as cells begin DNA replication.About:
This article is published in Journal of Biological Chemistry.The article was published on 1988-06-15 and is currently open access. It has received 440 citations till now. The article focuses on the topics: Thymidine kinase activity & Thymidine kinase 1.read more
Citations
More filters
Journal ArticleDOI
G1 events and regulation of cell proliferation.
TL;DR: This work has shown that switches in and out of G1 are the main determinants of post-embryonic cell proliferation rate and are defectively controlled in cancer cells.
Journal ArticleDOI
Imaging proliferation in vivo with [F-18]FLT and positron emission tomography.
Anthony F. Shields,John R. Grierson,B M Dohmen,Hans Jürgen Machulla,J C Stayanoff,Jawana M. Lawhorn-Crews,Joyce E. Obradovich,Otto Muzik,Thomas J. Mangner +8 more
TL;DR: [F-18]FLT (3'-deoxy-3'-fluorothymidine) is developed and tested; it is resistant to degradation, is retained in proliferating tissues by the action of thymidine kinase 1 (TK), and produces high-contrast images of normal marrow and tumors in canine and human subjects.
Journal ArticleDOI
Mammalian Deoxyribonucleoside Kinases
TL;DR: The physiologic metabolic role of the anabolic enzymes is discussed in relation to catabolic pathways, and alternative pathways for nucleoside analogue phosphorylation are surveyed, such as the phosphotransfer capacity of 5'-nucleotidase.
Journal ArticleDOI
Cellular localization and cell cycle regulation by a temperature-sensitive p53 protein
TL;DR: Primary rat embryo fibroblasts were transformed by a p53 mutant (alanine to valine change at amino acid 135) plus ras, and the S-phase cells appear to be immune to the p53 negative regulation of growth until they enter the next G1 period.
Journal ArticleDOI
Regulation of proliferating cell nuclear antigen during the cell cycle.
TL;DR: It is concluded that the cyclic synthesis of PCNA in cycling HeLa cells maintainsPCNA in excess of the amount involved directly in DNA replication and the amount of the protein neither fluctuates significantly with the cell cycle nor is limiting for DNA synthesis.
References
More filters
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
Journal ArticleDOI
Cyclin: A protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division
TL;DR: Eggs of the sea urchin Lytechinus pictus and oocytes of the surf clam Spisula solidissima also contain proteins that only start to be made after fertilization and are destroyed at certain points in the cell division cycle, and it is proposed to call these proteins the cyclins.
Journal ArticleDOI
The clam embryo protein cyclin A induces entry into M phase and the resumption of meiosis in Xenopus oocytes
Katherine I. Swenson,Katherine I. Swenson,Kevin M. Farrell,Kevin M. Farrell,Joan V. Ruderman,Joan V. Ruderman +5 more
TL;DR: Findings indicate that the rise in cyclin A plays a direct and natural role in driving cells into M phase.