Relation Between Neutralization of Epstein-Barr Virus and Antibodies to Cell-Membrane Antigens Induced by the Virus
01 Nov 1970-Journal of the National Cancer Institute (Oxford University Press)-Vol. 45, Iss: 5, pp 989-995
TL;DR: The neutralization of Epstein-Barr virus infectivity by sera with known antibody activities against EBV-associated antigens was investigated in experimentally infected Raji and RPMI 64-10 cells and the implication of these results on the relationship of the membraneAntigens to the infectious virus particle is discussed.
Abstract: The neutralization of Epstein-Barr virus (EBV) infectivity by sera with known antibody activities against EBV-associated antigens was investigated in experimentally infected Raji and RPMI 64-10 cells. Antibodies against EBV-induced cell-membrane antigens were apparently responsible for neutralization of viral infectivity. Antibodies against EBV capsid antigens and EBV-induced early antigens were not involved. The implication of these results on the relationship of the membrane antigens to the infectious virus particle is discussed.
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TL;DR: This review of the life cycle of the Epstein–Barr virus explains how EBV establishes lifelong infection in a host with protective immunity against the virus.
Abstract: This review of the life cycle of the Epstein–Barr virus (EBV) explains how EBV establishes lifelong infection in a host with protective immunity against the virus. The authors also discuss the role of EBV in the development of post-transplantation lymphoma, Hodgkin's disease, and Burkitt's lymphoma.
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TL;DR: Results obtained in a recent series of 90 patients with infectious mononucleosis are presented to show that an Epstein-Barr virus specific serodiagnosis can be established in most, if not all, cases, provided the most pertinent tests are performed.
500 citations
TL;DR: Human peripheral lymphocytes were investigated for receptors binding Epstein-Barr virus because of the regular association of this virus with infectious mononucleosis and Burkitt's lymphoma and it was concluded that B lymphocytes, in contrast to T lymphocyte, have receptors for EBV.
Abstract: Human peripheral lymphocytes were investigated for receptors binding Epstein-Barr virus (EBV) because of the regular association of this virus with infectious mononucleosis and Burkitt's lymphoma. This was done by a cytoadherence technique where virus-producing cells, displaying fresh viral determinants in their cytoplasmatic membrane, were mixed with lymphocytes. Unfractionated lymphocytes were found to adhere to these cells in contrast to column-purified T lymphocytes. The specificity of the binding was confirmed by blocking experiments that showed that sera containing high titers of antibodies directed against the virus could partially inhibit the adherence in contrast to low-titer sera. It is concluded that B lymphocytes, in contrast to T lymphocytes, have receptors for EBV. In a second line of experiments it was found that established human lymphoblastoid lines that carry the EBV genome had receptors characteristic for B lymphocytes and did not form T-lymphocyte rosettes. In contrast, a line of known T-lymphocyte origin that did not carry the EBV genome had receptors characteristic for T lymphocytes. EBV-transformed simian lymphoblastoid lines had surface markers indicating a B-lymphocyte origin in contrast to HVS-transformed simian lines that lacked surface immunoglobulin but carried receptors for sheep red blood cells.
498 citations
Cites background from "Relation Between Neutralization of ..."
...They share antigenic specificity(ies) as MA, the EBV-induced membrane antigen (18, 19), can can be demonstrated by staining live P3HR-1 cell suspensions with anti-MA-positive, fluorescein-conjugated human IgG (17)....
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TL;DR: The biology of EBV and the host immune response are described and the characteristics and pathogenesis of infectious mononucleosis are discussed in the context of developing therapeutic and preventative strategies.
Abstract: Summary: Epstein-Barr virus (EBV) is a gammaherpesvirus that infects a large fraction of the human population. Primary infection is often asymptomatic but results in lifelong infection, which is kept in check by the host immune system. In some cases, primary infection can result in infectious mononucleosis. Furthermore, when host-virus balance is not achieved, the virus can drive potentially lethal lymphoproliferation and lymphomagenesis. In this review, we describe the biology of EBV and the host immune response. We review the diagnosis of EBV infection and discuss the characteristics and pathogenesis of infectious mononucleosis. These topics are approached in the context of developing therapeutic and preventative strategies.
347 citations
Cites background from "Relation Between Neutralization of ..."
...The EBV envelope glycoprotein, gp350 (formerly known as gp340 or EBV-induced cell membrane antigen), has been considered an attractive immunogen ever since it was shown to neutralize the virus (156)....
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