Open AccessJournal Article
Relationship between DNA damage and sperm head birefringence
Claudia G. Petersen,Laura D. Vagnini,C. M. Junta,A.L. Mauri,Fabiana C. Massaro,L. F. I. Silva,J. Ricci,Mario Cavagna,Anaglória Pontes,R. L. R. Baruffi,J. B. A. Oliveira,J.G. Franco +11 more
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TLDR
The data support a positive relationship between spermatozoa with total SHBF in their head and increased DNA fragmentation.Abstract:
Sao Paulo State Univ UNESP, Botucatu Med Sch, Ctr Human Reprod Prof Franco Jr,Res Unit, Paulista Ctr Diag Res & Training,Dept Gynecol & O, Ribeirao Preto, Brazilread more
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Sperm preparation: state-of-the-art--physiological aspects and application of advanced sperm preparation methods.
TL;DR: Techniques in this category are magnet-activated cell sorting, Annexin V-activated glass wool filtration, flow cytometry and picked spermatozoa for ICSI (PICSI) from HA-coated dishes and HA-containing media.
Journal ArticleDOI
Sperm processing for advanced reproductive technologies: Where are we today?
Kari Rappa,Harold F. Rodriguez,Gloria C. Hakkarainen,Raymond M. Anchan,George L. Mutter,Waseem Asghar +5 more
TL;DR: Some recent research in centrifugation and non-centrifugation based techniques and their effect on sperm quality and ART outcomes are discussed.
Sperm chromatin structure and male fertility:biological and clinical aspects
TL;DR: Evaluation of sperm DNA damage appears to be a useful tool for assessing male fertility potential both in vivo and in vitro.
Journal ArticleDOI
Novel technologies for selecting the best sperm for in vitro fertilization and intracytoplasmic sperm injection
TL;DR: The research evidence that supports the interrelated developmental and genetic integrity of the selected sperm, particularly sperm DNA damage and clinical outcome evidence are presented.
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Sperm selection in assisted reproduction: A review of established methods and cutting-edge possibilities
Giuseppina Marzano,Maria Serena Chiriacò,Elisabetta Primiceri,Maria Elena Dell'Aquila,João Ramalho-Santos,Vincenzo Zara,Alessandra Ferramosca,Giuseppe Maruccio +7 more
TL;DR: The techniques routinely performed in human and animal clinical practice for sorting good-quality sperm for in vitro fertilization procedures are described, and the positive and negative aspects of each method are focused on.
References
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Journal ArticleDOI
On the possible origins of DNA damage in human spermatozoa
TL;DR: A two-step hypothesis for the origins of DNA damage in human spermatozoa is presented that highlights the significance of oxidative stress acting on vulnerable, poorly protaminated cells generated as a result of defective spermiogenesis.
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A test for the practical evaluation of male fertility by acridine orange (AO) fluorescence.
TL;DR: In this article, the authors introduced the concept of an effective sperm count, which is obtained by multiplying the percentage of green-fluorescing sperm by the actual sperm count and found that the percent green correlates with neither actual sperm counts nor motility, indicating that this test measures a new parameter of male fertility.
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Late, but not early, paternal effect on human embryo development is related to sperm DNA fragmentation
TL;DR: Evaluation of sperm DNA integrity is useful to detect late paternal effect, which is not associated with morphological abnormalities at the zygote and early cleavage stages, which can compromise ART outcomes in the absence of increased sperm DNA fragmentation.
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Influence of deoxyribonucleic acid damage on fertilization and pregnancy
Ralf Henkel,Marjam Hajimohammad,T. Stalf,Christiaan F. Hoogendijk,Claas Mehnert,Roelof Menkveld,H. Gips,Wolf-Bernhard Schill,Thinus F. Kruger +8 more
TL;DR: The data clearly demonstrate that DNA fragmentation, as determined by the TUNEL assay, is predictive for pregnancy in IVF, which implies that spermatozoa with DNA fragmentation can still fertilize an oocyte but that when paternal genes are "switched on," further embryonic development stops, resulting in failed pregnancy.
Breakthroughs in Andrology Real-Time Fine Morphology of Motile Human Sperm Cells is Associated With IVF-ICSI Outcome
TL;DR: Findings indicate that ICSI-associated pregnancy rate may be affected by subtle morphological malformations of the sperm nucleus, which may remain undetected by the embryologist during the routine selection procedure.