Journal ArticleDOI
Repair of x-ray damage in DNA of cultivated cells from patients having xeroderma pigmentosum.
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TLDR
Results suggest that the xeroderma strains investigated were deficient in the enzyme(s) involved in the excision of pyrimidine dimers from the DNA.Abstract:
Repair replication and rejoining of single-strand breaks after X-irradiation in human-skin fibroblasts from normal donors and several patients with xeroderma pigmentosum have been compared. The xeroderma strains showed different levels of repair replication following UV exposure. Repair replication and rejoining of breaks, which are considered to be part of the repair mechanism after damage due to X-irradiation and UV-irradiation, appeared to be performed in all xeroderma pigmentosum strains tested to the same level as in control strains. These results, and the observation that in the same xeroderma pigmentosum strains repair replication after UV irradiation was considerably reduced, suggest that the xeroderma strains investigated were deficient in the enzyme(s) involved in the excision of pyrimidine dimers from the DNA.read more
Citations
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Ataxia telangiectasia: a human mutation with abnormal radiation sensitivity
A. M. R. Taylor,D. G. Harnden,Colin F. Arlett,Susan A. Harcourt,Alan R. Lehmann,S. Stevens,Bryn A. Bridges +6 more
TL;DR: Cell survival experiments are reported which indicate that the clinically observed enhanced sensitivity of AT patients to ionising radiation is manifest at the cellular level.
Journal ArticleDOI
Xeroderma pigmentosum. An inherited diseases with sun sensitivity, multiple cutaneous neoplasms, and abnormal DNA repair.
Journal ArticleDOI
The origin of human cancers.
TL;DR: It is shown that most human cancers are not caused by conventional mutagens but are more likely to be the result of genetic transpositions, and the external factors that influence its frequency have not yet been studied in any detail.
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Genetic heterogeneity of xeroderma pigmentosum demonstrated by somatic cell hybridization.
TL;DR: Using the dark repair mechanism in microorganisms as a model, evidence has been presented that XP cells are defective in the incision step of DNA repair3–5.
Journal ArticleDOI
Xeroderma Pigmentosum: Variants with Normal Dna Repair and Normal Sensitivity to Ultraviolet Light
TL;DR: The subjects are three patients with distinct symptoms of xeroderma pigmentosum in which the cultured fibroblasts are different from those usually found in this disease, and a minority of those cases which are clinically diagnosed as XP constitute a biochemically distinct condition.
References
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Journal ArticleDOI
Sedimentation studies of the size and shape of dna.
TL;DR: Analytical zone sedimentation ( Vinograd, Bruner, Kent & Weigle, 1963) is shown to be a sensitive and reliable method for detecting differences in conformation and for determining the molecular weight of homogeneous phage DNA's.
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Defective repair replication of DNA in xeroderma pigmentosum.
TL;DR: Patients with xeroderma pigmentosum develop fatal skin cancers when exposed to sunlight, and so the failure of DNA repair in the skin must be related to carcinogenesis.
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Xeroderma pigmentosum: a human disease in which an initial stage of dna repair is defective
TL;DR: Homozygous xeroderma pigmentosum fibroblasts cannot repair damage to DNA bases, but can repair damage that involves chain breaks, and may be the result of somatic mutations caused by unrepaired damage.
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Evidence that ultraviolet-induced thymine dimers in dna cause biological damage
Richard B. Setlow,Jane K. Setlow +1 more
TL;DR: 6 Lengyel, P., J. F., P. F. Speyer, C. Basilio, and S. Ochoa, these PROCEEDINGS, 48, 282 (1962).
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Rejoining of x-ray induced breaks in the DNA of leukaemia cells.
TL;DR: The number of breaks in the polynucleotide chains of the DNA in murine leukaemia cells, the bacterium Micrococcus radiodurans and in Isolated DNA are approximately the same and the results suggest that the molecular weight of the mammalian DNA is very high.