Replication of Subgenomic Hepatitis C Virus RNAs in a Hepatoma Cell Line
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Additional excerpts
...(d) Immunofluorescence assay of cells fixed 72 h after transfection with JFH1 (1–3) or JFH1/∆E1-E2 RNA (4)....
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...Core protein was secreted efficiently from JFH1 RNA but much less efficiently from JFH1/ΔE1-E2 RNA–transfected cells (Fig....
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...Cells transfected with JFH1/ΔE1-E2 RNA or a subgenomic replicon (SGR-JFH1)8 lacking the core to NS2 region served as controls....
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...(b) Comparison of infectivity of culture supernatant from JFH1 RNA– and JFH1/ΔE1-E2 RNA– transfected cells....
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...To compare infectivity of culture supernatant of JFH1 RNA– and JFH1/ΔE1-E2 RNA– transfected cells, we inoculated Huh7 cells with concentrates containing equivalent RNA copy numbers (1.17 × 108 copies/ml)....
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Cites background from "Replication of Subgenomic Hepatitis..."
...Negative-strand RNA then serves as a template for multiple rounds of nascent, positivestrand synthesis, leading to the asymmetric accumulation of nearly 10 positive-strands for every negative strand (7,375,429,491,583)....
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...strain with the neomycin-resistance gene (429)....
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1,766 citations
References
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