Journal ArticleDOI
Reproducible Imaging and Dissection of Plasmid DNA Under Liquid with the Atomic Force Microscope
Helen G. Hansma,James Vesenka,C. Siegerist,G.L. Kelderman,H. Morrett,Robert L. Sinsheimer,V. Elings,Carlos Bustamante,Paul K. Hansma +8 more
Reads0
Chats0
TLDR
Reproducible images of uncoated DNA in the atomic force microscope (AFM) have been obtained by imaging plasmid DNA on mica in n-propanol by increasing the force applied by the AFM tip at selected locations.Abstract:
Reproducible images of uncoated DNA in the atomic force microscope (AFM) have been obtained by imaging plasmid DNA on mica in n-propanol. Specially sharpened AFM tips give images with reproducible features several nanometers in size along the DNA. Plasmids can be dissected in propanol by increasing the force applied by the AFM tip at selected locations.read more
Citations
More filters
Journal ArticleDOI
Design and self-assembly of two-dimensional DNA crystals
TL;DR: The design and observation of two-dimensional crystalline forms of DNA that self-assemble from synthetic DNA double-crossover molecules that create specific periodic patterns on the nanometre scale are reported.
Journal ArticleDOI
From molecules to cells: imaging soft samples with the atomic force microscope
TL;DR: An overview is presented on the application of atomic force microscopy to organic samples ranging from thin ordered films at molecular resolution to living cells and novel imaging modes are introduced that exploit different aspects of the tip-sample interaction for local measurements of the micromechanical properties of the sample.
Journal ArticleDOI
Imaging modes of atomic force microscopy for application in molecular and cell biology
Yves F. Dufrêne,Toshio Ando,Ricardo Garcia,David Alsteens,David Martinez-Martin,Andreas Engel,Christoph Gerber,Daniel J. Müller +7 more
TL;DR: The basic principles, advantages and limitations of the most common AFM bioimaging modes are reviewed, including the popular contact and dynamic modes, as well as recently developed modes such as multiparametric, molecular recognition, multifrequency and high-speed imaging.
Proceedings ArticleDOI
Algorithmic Self-Assembly of DNA
TL;DR: In this paper, the authors focus on molecular self-assembly, giving examples of engineered DNA tiles that crystallize into two-dimensional sheets, one-dimensional tubes and ribbons, and information-guided patterns such as a Sierpinski triangle and a binary counter.
Journal ArticleDOI
Biomolecular Imaging with the Atomic Force Microscope
Helen G. Hansma,Jan H. Hoh +1 more
References
More filters
Journal ArticleDOI
Atomic force microscope
TL;DR: The atomic force microscope as mentioned in this paper is a combination of the principles of the scanning tunneling microscope and the stylus profilometer, which was proposed as a method to measure forces as small as 10-18 N. As one application for this concept, they introduce a new type of microscope capable of investigating surfaces of insulators on an atomic scale.
Journal ArticleDOI
Imaging crystals, polymers, and processes in water with the atomic force microscope.
Barney Drake,Craig Prater,A. L. Weisenhorn,Scot A. C. Gould,T. R. Albrecht,Calvin F. Quate,David S. Cannell,Helen G. Hansma,Paul K. Hansma +8 more
TL;DR: Images of mica demonstrate that atomic resolution is possible on rigid materials, thus opening the possibility of atomic-scale corrosion experiments on nonconductors and showing the potential of the AFM for revealing the structure of molecules important in biology and medicine.
Journal ArticleDOI
Forces in atomic force microscopy in air and water
TL;DR: In this article, an atomic force microscope with an optical lever detection system was used to measure the absolute force applied by a tip on a surface, which can be as low as 10−9 N or less in water and 10−7 N in air.
Journal ArticleDOI
Atomic Resolution with Atomic Force Microscope
TL;DR: The atomic force microscope (AFM) is a promising new method for studying the surface structure of both conductors and insulators as discussed by the authors, achieving a resolution better than 2.5 A.