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Retinal function in an isolated, perfused mammalian eye.
Peter Gouras,Mary Hoff +1 more
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In this preparation, rod function is more vulnerable to damage than cone function, and this vidnerability appears to occur somewhere between the rod receptor cell and second-order neurons in the retina.Abstract:
Retinal function has been examined by identifying the activity of both rod and cone receptor systems in the electroretinogram (ERG) and the response of the optic nerve in the isolated cat eye, perfused through its ophthalmociliary artery with a solution devoid of hemoglobin. This preparation can maintain an ERG of normal configuration and of relatively large amplitude, dark-adapts after strong adapting lights, shows a Purkinje shift, and has synoptic transmission from the receptors up to and including the ganglion cell layer of the retina. In this preparation, rod function is more vulnerable to damage than cone function, and this vidnerability appears to occur somewhere between the rod receptor cell and second-order neurons in the retina.read more
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Intracellular staining reveals different levels of stratification for on- and off-center ganglion cells in cat retina
TL;DR: No other morphological feature except stratification appears to be important in determining the sign of the response of these cells, and the nature of the rod and cone pathways to ganglion cells is discussed.
Journal ArticleDOI
Horizontal cells in cat retina with independent dendritic systems
TL;DR: Cat horizontal cells are retinal neurons with two functionally distinct parts: the cell body receives signals predominantly from cones, while the terminal arborization receives predominantly from rods.
Journal ArticleDOI
The function of the retina in the perfused eye
TL;DR: The data show that the enucleated eye of the cat can be maintained in apparently physiologically functioning condition by appropriate arterial perfusion, and since movements of cardiovascular and respiratory origin, invariably present to varying extent in thein vivo experiments, are eliminated, this preparation is suitable for intracellular recordings from neuronal elements of the retina.
Journal ArticleDOI
Retinal capillary perfusion: Spatial and temporal heterogeneity.
Dao-Yi Yu,Stephen J. Cringle,Paula K. Yu,Chandrakumar Balaratnasingam,Andrew Mehnert,Marinko V. Sarunic,Dong An,Er-Ning Su +7 more
TL;DR: There is significant and necessary spatial and temporal heterogeneity and active regulation of retinal blood flow in the retina, particularly in the macular region, and how such a delicate balance of metabolic supply and consumption is achieved is considered.
References
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Journal ArticleDOI
Amino Acid Metabolism in Mammalian Cell Cultures
TL;DR: The present article "is a progress report rather than a review and in large part summarizes studies from a single laboratory" on the minimal essential medium for cultivation of mammalian cells in either monolayer or suspension.
Journal ArticleDOI
Organization of the Primate Retina: Light Microscopy
TL;DR: The structure of the human, but mainly of the rhesus monkey, retina as examined by Golgi-staining techniques is described and interpreted on evidence from both light and electron microscopy.
BookDOI
Recollections of My Life
TL;DR: Almost every important conceptual issue in neurobiology was foreshadowed in Cajal's work: the initial description of the climbing fibers of the cerebellum, the discovery of the growth cone, the concept of the "dynamic polarity" of the neurom an anticipation of the later discovery of axonal transport, and the prediction that new synapses may be formed throughout life to serve as a physical basis for learning and memory.
Journal ArticleDOI
Rod and cone interaction in dark-adapted monkey ganglion cells
P. Gouras,Krista Link +1 more
TL;DR: Rod—cone interaction has been studied by analysing the response latency of large ganglion cells in the perifovea of dark‐adapted Rhesus monkey retina with good results for rod-cone interaction.
Journal ArticleDOI
Light and dark adaptation in the isolated rat retina.
TL;DR: A living isolated retina exhibits the initial fast phase, but not the later slow phase of dark adaptation, which is directly related to the bleaching of rhodopsin.