Journal ArticleDOI
Reversing an Extracellular Electron Transfer Pathway for Electrode-Driven Acetoin Reduction
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TLDR
This system uses native Mtr proteins to transfer electrons from an electrode to the inner membrane quinone pool and uses reduction of acetoin to 2,3-butanediol via a heterologous butanediol dehydrogenase (Bdh) as an electron sink.Abstract:
Microbial electrosynthesis is an emerging technology with the potential to simultaneously store renewably generated energy, fix carbon dioxide, and produce high-value organic compounds. However, limited understanding of the route of electrons into the cell remains an obstacle to developing a robust microbial electrosynthesis platform. To address this challenge, we leveraged the native extracellular electron transfer pathway in Shewanella oneidensis MR-1 to connect an extracellular electrode with an intracellular reduction reaction. The system uses native Mtr proteins to transfer electrons from an electrode to the inner membrane quinone pool. Subsequently, electrons are transferred from quinones to NAD+ by native NADH dehydrogenases. This reverse functioning of NADH dehydrogenases is thermodynamically unfavorable; therefore, we added a light-driven proton pump (proteorhodopsin) to generate proton-motive force to drive this activity. Finally, we use reduction of acetoin to 2,3-butanediol via a heterologous butanediol dehydrogenase (Bdh) as an electron sink. Bdh is an NADH-dependent enzyme; therefore, observation of acetoin reduction supports our hypothesis that cathodic electrons are transferred to intracellular NAD+. Multiple lines of evidence indicate proper functioning of the engineered electrosynthesis system: electron flux from the cathode is influenced by both light and acetoin availability, and 2,3-butanediol production is highest when both light and a poised electrode are present. Using a hydrogenase-deficient S. oneidensis background strain resulted in a stronger correlation between electron transfer and 2,3-butanediol production, suggesting that hydrogen production is an off-target electron sink in the wild-type background. This system represents a promising step toward a genetically engineered microbial electrosynthesis platform and will enable a new focus on synthesis of specific compounds using electrical energy.read more
Citations
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Journal ArticleDOI
Fundamentals, Applications, and Future Directions of Bioelectrocatalysis.
Hui Chen,Olja Simoska,Koun Lim,Matteo Grattieri,Mengwei Yuan,Fangyuan Dong,Yoo Seok Lee,Kevin Beaver,Samali Weliwatte,Erin M. Gaffney,Shelley D. Minteer +10 more
TL;DR: This review seeks to systematically and comprehensively detail the fundamentals, analyze the existing problems, summarize the development status and applications, and look toward the future development directions of bioelectrocatalysis.
Journal ArticleDOI
Strategies for improving the electroactivity and specific metabolic functionality of microorganisms for various microbial electrochemical technologies
P. Chiranjeevi,Sunil A. Patil +1 more
TL;DR: The major strategies that are used to enhance the electroactivity and specific functionality of microorganisms pertaining to both anodic and cathodic processes of METs are presented.
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Microbial electro-fermentation for synthesis of chemicals and biofuels driven by bi-directional extracellular electron transfer.
TL;DR: This review demonstrates the main bi-directional EET mechanisms during EF, including the direct EET and the shuttle-mediated EET, and summarizes the main synthetic biology strategies in improving the bi- Directional E ET and target products synthesis to enhance the efficiencies in unbalanced fermentation and microbial electrosynthesis.
Journal ArticleDOI
Photoferrotrophs Produce a PioAB Electron Conduit for Extracellular Electron Uptake.
Dinesh Gupta,Molly C. Sutherland,Karthikeyan Rengasamy,J. Mark Meacham,Robert G. Kranz,Arpita Bose +5 more
TL;DR: The presence of PioAB in these organisms correlate with their ability to perform photoferrotrophy and phototrophic EEU because of its conserved characteristics in phototrophs harboring homologous proteins.
Journal ArticleDOI
Degradation of organic contaminants and steel corrosion by the dissimilatory metal-reducing microorganisms Shewanella and Geobacter spp.
Zhou Jiang,Meimei Shi,Liang Shi +2 more
TL;DR: Current understandings of extracellular electron transfer mechanisms of as well as degradation of organic contaminants and steel corrosion by Shewanella and Geobacter spp.
References
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Journal ArticleDOI
Microbial electrosynthesis — revisiting the electrical route for microbial production
Korneel Rabaey,René A. Rozendal +1 more
TL;DR: This Review addresses the principles, challenges and opportunities of microbial electrosynthesis, an exciting new discipline at the nexus of microbiology and electrochemistry.
Journal ArticleDOI
Bacterial Manganese Reduction and Growth with Manganese Oxide as the Sole Electron Acceptor
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