RNA as a tumor vaccine: a review of the literature
Citations
384 citations
Cites background from "RNA as a tumor vaccine: a review of..."
...Despite the fact that mRNA transfection was successfully used for ectopic gene expression in mammalian cells (4), the initial reports describing the immunostimulatory capacity of mRNA-transfected DCs were met with healthy skepticism, reflecting to some extent a myth that RNA is extremely labile and could not withstand the transfection protocols....
[...]
289 citations
Cites background from "RNA as a tumor vaccine: a review of..."
...Moreover, when considering vaccination against infectious diseases, genetic vaccinations eliminate the risk of mutation and uncontrollable proliferation of inactivated pathogens [109]....
[...]
208 citations
194 citations
191 citations
References
4,872 citations
"RNA as a tumor vaccine: a review of..." refers background in this paper
...Dendritic cells (DCs) are known to have a crucial role in the activation of T- and Bcell immunity (10)....
[...]
4,022 citations
"RNA as a tumor vaccine: a review of..." refers background in this paper
...The first indication that mRNA could be used without a protective liposome came in 1990 when Wolff and colleagues (42) injected naked mRNA for luciferase, ß-galactosidase and chloramphenicol acetyltransferase directly into muscle and demonstrated gene expression for several days....
[...]
...The translation of exogenous RNA into antigen has been shown in dendritic cells (59) and in myocytes (42, 43)....
[...]
...ELISA testing of antigen production (38), b-gal tissue staining (8), luciferase activity measurement (42) have all shown that injected mRNA for a particular protein results in increased production of that specific protein....
[...]
2,993 citations
2,844 citations
Additional excerpts
...The use of GM-CSF as an adjuvant to peptide and DNA vaccines has been shown to have an enhancing anti-tumor effect in human and mice tumor models (50, 52)....
[...]
2,437 citations
"RNA as a tumor vaccine: a review of..." refers background in this paper
...Previous studies have shown that apoptotic cells are more readily taken up by DCs (44)....
[...]