RNA-Seq: a revolutionary tool for transcriptomics
Citations
151 citations
Cites background from "RNA-Seq: a revolutionary tool for t..."
...Examples of this include expression of unknown target sequences, RNA editing events and other RNA sequence variations such as polymorphisms [21,22,23]....
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...This is partly due to the greatly increased dynamic range for quantification of gene expression provided by the high-throughput sequencing method [22]....
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151 citations
Cites background or methods from "RNA-Seq: a revolutionary tool for t..."
...Even though, initially microarray has been instrumental in whole transcriptome analysis, currently RNA-seq is becoming a preferred method of choice, since it is considered to effectively surmount the limitations of microarray [1,21-23]....
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...Currently several studies have been conducted to compare the performance of RNA-seq and microarray in quantifying the expression level of genes, by focusing on various aspects like reproducibility, accuracy, statistical issues, technical and biological variabilities [1,15,21,27-30]....
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...The sheer ability to simultaneously quantify the expression levels for a vast number of genes has revolutionized the biomedical research, facilitating the analysis of global gene expression patterns at the genome-wide scale [1]....
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...; licensee BioMed Cent Commons Attribution License (http://creativec reproduction in any medium, provided the or profiling methods, RNA-seq and DNA microarray stand out as the two widely used genome-wide gene expression quantification methods [1-17]....
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...Further, RNA-seq data contains very low background signal, a higher dynamic range of expression levels, and also relatively small amount of total RNA required for quantification, when compared to microarray [1,23]....
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151 citations
Cites background from "RNA-Seq: a revolutionary tool for t..."
...Background Deep transcriptome sequencing (RNA-Seq) with nextgeneration sequencing (NGS) technologies is providing unprecedented opportunities for researchers to probe the transcriptomes of many species [1-5]....
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151 citations
151 citations
Cites methods or result from "RNA-Seq: a revolutionary tool for t..."
...Probably due to this very high depth of sequencing, expression of most PsCam_LowCopy contigs was detected in almost all plant tissues (Figure 3), as previously reported in other experiments (Wang et al., 2009), and more than 80% of contigs appeared to be expressed in each library (Figure 3)....
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...RNA-seq has been described as a very robust and sensitive tool for transcriptomics (’t Hoen et al., 2008; Wang et al., 2009; Garg and Jain, 2013)....
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References
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"RNA-Seq: a revolutionary tool for t..." refers methods in this paper
...There are several programs for mapping reads to the genome, including ELAND, SOA...
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