RNA-Seq: a revolutionary tool for transcriptomics
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TLDR
The RNA-Seq approach to transcriptome profiling that uses deep-sequencing technologies provides a far more precise measurement of levels of transcripts and their isoforms than other methods.Abstract:
RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.read more
Citations
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Plant-associated fungal communities in the light of meta’omics
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Strand-specific libraries for high throughput RNA sequencing (RNA-Seq) prepared without poly(A) selection
TL;DR: The RNA-Seq protocol described here yields strand-specific transcriptome libraries without poly(A) selection, which provide approximately 90% mappable sequences.
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The Transcriptomes of Two Heritable Cell Types Illuminate the Circuit Governing Their Differentiation
Brian B. Tuch,Quinn M. Mitrovich,Oliver R. Homann,Aaron D. Hernday,Cinna Monighetti,Francisco M. De La Vega,Alexander D. Johnson +6 more
TL;DR: The revised Wor1 circuit bears several striking similarities to the Oct4 circuit that specifies the pluripotency of mammalian embryonic stem cells, which suggest a set of general hallmarks characteristic of heritable differentiation states in eukaryotes.
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Impacts of variation in the human genome on gene regulation.
TL;DR: The current state of how genetic variations impact gene regulation locally and globally in the human genome is reviewed.
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The core promoter: At the heart of gene expression.
TL;DR: A broad spectrum of studies that highlight the importance of the core promoter and its pivotal role in the regulation of metazoan gene expression are reviewed and future research directions and challenges are suggested.
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