RNA-Seq: a revolutionary tool for transcriptomics
Citations
138 citations
Cites methods from "RNA-Seq: a revolutionary tool for t..."
...Subsequently, each cDNA molecule is sequenced by high-throughput sequencing technology to obtain short sequences of sizes ranging from 30 to 400 bp depending upon the sequencing technology used [21]....
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138 citations
Cites background or methods from "RNA-Seq: a revolutionary tool for t..."
...High-throughput RNA sequencing, also known as RNA-seq, is becoming a popular technique to measure gene expression abundance (Mortazavi et al., 2008; Wang, Gerstein, and Snyder, 2009)....
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...For example, RNA-seq is less noisy and has a much larger dynamic range than microarrays, and RNA-seq can identify new transcripts whereas microarray’s detection capability is limited by the probes on the array (Wang et al., 2009)....
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138 citations
138 citations
Cites background or methods from "RNA-Seq: a revolutionary tool for t..."
...In this study, we applied the RNA-seq technology and revealed the transcriptomes of bovine in vivo pre-implantation development in a very high-throughput and quantitative manner [14]....
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...The RNA-seq technology provides unique benefits for studying gene expression with high resolutions and reproducibility, as well as for detecting novel transcripts and alternative splicing events [14,15]....
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138 citations
Cites background from "RNA-Seq: a revolutionary tool for t..."
...Actually, the newest methodological advance in this direction consists of the deep sequencing of RNA (RNA-Seq) that is allowing the identification and quantification of the transcriptome, including those of nonmodel organisms (Wang et al. 2009b and reference therein)....
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References
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"RNA-Seq: a revolutionary tool for t..." refers methods in this paper
...There are several programs for mapping reads to the genome, including ELAND, SOA...
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