RNA-Seq: a revolutionary tool for transcriptomics
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TLDR
The RNA-Seq approach to transcriptome profiling that uses deep-sequencing technologies provides a far more precise measurement of levels of transcripts and their isoforms than other methods.Abstract:
RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.read more
Citations
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Comparative Evolutionary and Developmental Dynamics of the Cotton (Gossypium hirsutum) Fiber Transcriptome
Mi-Jeong Yoo,Jonathan F. Wendel +1 more
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Characterization of duplicate gene evolution in the recent natural allopolyploid Tragopogon miscellus by next-generation sequencing and Sequenom iPLEX MassARRAY genotyping.
Richard J. A. Buggs,Richard J. A. Buggs,Srikar Chamala,Wei Wu,L. Gao,G. D. May,Patrick S. Schnable,Douglas E. Soltis,Pamela S. Soltis,Pamela S. Soltis,William B. Barbazuk +10 more
TL;DR: Using 454 and Illumina expressed sequence tag sequencing of the parental diploid species of T. miscellus, 7782 single nucleotide polymorphisms that differ between the two progenitor genomes present in this allotetraploid are identified.
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Next-generation sequencing in aging research: emerging applications, problems, pitfalls and possible solutions
TL;DR: An overview of state-of-the-art next-generation sequencing (NGS) platforms and their applications, including in genome sequencing and resequencing, transcriptional profiling and high-throughput survey of DNA-protein interactions (ChIP-Seq) and of the epigenome is provided.
Journal ArticleDOI
3'-end sequencing for expression quantification (3SEQ) from archival tumor samples.
Andrew H. Beck,Ziming Weng,Daniela Witten,Shirley Zhu,Joseph W. Foley,Phil Lacroute,Cheryl L. Smith,Robert Tibshirani,Matt van de Rijn,Arend Sidow,Robert B. West,Robert B. West +11 more
TL;DR: It is demonstrated that 3SEQ is an effective technique for gene expression profiling from archival tumor samples and may facilitate significant advances in translational cancer research.
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Trends in Next-Generation Sequencing and a New Era for Whole Genome Sequencing.
Sang Tae Park,Jayoung Kim +1 more
TL;DR: One of the most popular NGS applications, whole genome sequencing (WGS), developed from the expansion of human genomics is introduced and a general overview for next-generation sequencing (NGS) is provided.
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