RNA-Seq: a revolutionary tool for transcriptomics
Citations
102 citations
Cites background from "RNA-Seq: a revolutionary tool for t..."
...Using this approach, one can achieve unprecedented levels of accuracy and specificity in quantifying differentially expressed genes, identifying novel transcribed regions, and identifying alternative splice events.(29,30) Using a high-throughput gene expression profiling technique and available complete soybean genome sequences, genes involved in the soybean response to Xag infection were identified by differential expression profiling....
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102 citations
102 citations
Cites background from "RNA-Seq: a revolutionary tool for t..."
...RNA-seq is a relatively new technique that can be used to analyse changes in gene expression across the entire transcriptome [13,14], and is now being applied to a rapidly increasing number of organisms [12]....
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102 citations
102 citations
Cites methods from "RNA-Seq: a revolutionary tool for t..."
...The advent of NGS technologies has spawned new approaches to exploring the transcriptome (eg RNA-Seq).(362,363) This method allows the study of the expression of mRNAs and non-coding RNAs, and is also able to detect and identify new transcripts (coding and non-coding) that have not been formally annotated....
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References
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"RNA-Seq: a revolutionary tool for t..." refers methods in this paper
...There are several programs for mapping reads to the genome, including ELAND, SOA...
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