RNA-Seq: a revolutionary tool for transcriptomics
Citations
101 citations
101 citations
100 citations
100 citations
Cites background from "RNA-Seq: a revolutionary tool for t..."
...Deep sequencing of the transcriptome (facilitated by rapid technological advances) (wang et al. 2009) and the considerable progress in genome-scale mapping of traitassociated loci (brought about by GwaS) (Hindorff et al. 2009) promise to advance our understanding of the genetic mechanisms…...
[...]
100 citations
Cites background or methods from "RNA-Seq: a revolutionary tool for t..."
...Because RNA fragmentation produces a more even sequence read distribution than cDNA fragmentation (Wang et al. 2009), the mRNA was first sheared into short fragments using divalent cations at 94 C for exactly 5 min before cDNA synthesis....
[...]
...…research in many areas, including resequencing, microRNA expression profiling, DNA methylation and de novo transcriptome sequencing for non-model organisms (Barakat et al. 2009; Hale et al. 2009; Meyer et al. 2009; Wang et al. 2009, 2010b, c; Sun et al. 2010; Huang et al. 2010; Wei et al. 2011)....
[...]
References
12,293 citations
4,437 citations
2,927 citations
2,834 citations
2,729 citations
"RNA-Seq: a revolutionary tool for t..." refers methods in this paper
...There are several programs for mapping reads to the genome, including ELAND, SOA...
[...]