RNA-Seq: a revolutionary tool for transcriptomics
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TLDR
The RNA-Seq approach to transcriptome profiling that uses deep-sequencing technologies provides a far more precise measurement of levels of transcripts and their isoforms than other methods.Abstract:
RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.read more
Citations
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Journal ArticleDOI
Unity in defence: honeybee workers exhibit conserved molecular responses to diverse pathogens
Vincent Doublet,Yvonne Poeschl,Andreas Gogol-Döring,Andreas Gogol-Döring,Cédric Alaux,Desiderato Annoscia,Christian Aurori,Seth M. Barribeau,Oscar C. Bedoya-Reina,Oscar C. Bedoya-Reina,Oscar C. Bedoya-Reina,Mark J. F. Brown,James C. Bull,Michelle L. Flenniken,David W. Galbraith,Elke Genersch,Sebastian Gisder,Ivo Grosse,Holly L. Holt,Holly L. Holt,Dan Hultmark,H. Michael G. Lattorff,H. Michael G. Lattorff,Yves Le Conte,Fabio Manfredini,Dino P. McMahon,Robin F. A. Moritz,Francesco Nazzi,Elina L. Niño,Elina L. Niño,Katja Nowick,Ronald P. van Rij,Robert J. Paxton,Robert J. Paxton,Christina M. Grozinger +34 more
TL;DR: This article performed a meta-analysis of multiple published and new transcriptomes using a newly developed bioinformatics approach that filters genes based on their expression profile across datasets, identifying common and unique molecular responses of a model host species, the honey bee (Apis mellifera), to its major pathogens and parasites.
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TL;DR: A comprehensive genome-wide survey of the developmental transcriptome of the human filarial parasite Brugia malayi finds that the transcriptional program has a number of stage-specific pathways activated during worm development.
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Nanopore native RNA sequencing of a human poly(A) transcriptome
Rachael E. Workman,Alison D. Tang,Paul S. Tang,Miten Jain,John R. Tyson,Philip C. Zuzarte,Timothy Gilpatrick,Roham Razaghi,Joshua Quick,Norah Sadowski,Nadine Holmes,Jaqueline Goes de Jesus,Karen Jones,Terrance P. Snutch,Nicholas J. Loman,Benedict Paten,Matthew Loose,Jared T. Simpson,Jared T. Simpson,Hugh E. Olsen,Angela N. Brooks,Mark Akeson,Winston Timp +22 more
TL;DR: This study focused on poly(A) RNA from the human cell line GM12878, generating 9.9 million aligned sequence reads, which had an aligned N50 length of 1294 bases, and a maximum aligned length of over 21,000 bases.
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