RNA-Seq: a revolutionary tool for transcriptomics
Citations
93 citations
Cites background from "RNA-Seq: a revolutionary tool for t..."
...It can distinguish between paralogous genes, detect and quantify transcripts of low or high abundance and identify transcript sequence polymorphisms and novel trans-splicing and splice isoforms [17]....
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93 citations
Cites background from "RNA-Seq: a revolutionary tool for t..."
...The advent of RNA-Seq provides a far more high-throughput and precise measurement of levels of transcripts and their isoforms than other methods [14]....
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...It has clear advantages over other approaches and is expected to revolutionize the manner in which eukaryotic transcriptomes are analyzed [14]....
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93 citations
93 citations
Cites methods from "RNA-Seq: a revolutionary tool for t..."
...Whole genome transcriptome analysis via high-throughput sequencing of cDNA libraries, called RNA-Seq, became available a few years ago and enabled the qualitative analysis of the RNA inventory of species as well as the quantitative analysis of differential transcript levels under various conditions.(39,40) The first RNA-Seq study with an archaeal species was performed for Methanosarcina mazei Gö1 under different nitrogen availabilities, leading to the identification of 242 intergenic and antisense sRNA, including six cis-antisense sRNAs overlapping with transposase genes and 40 sRNA candidates containing very short ORFs potentially encoding peptides smaller than 30 amino acids....
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92 citations
Cites background from "RNA-Seq: a revolutionary tool for t..."
...it also enable us to detect novel transcripts and alternative splicing events [58, 59]....
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References
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"RNA-Seq: a revolutionary tool for t..." refers methods in this paper
...There are several programs for mapping reads to the genome, including ELAND, SOA...
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