RNA-Seq: a revolutionary tool for transcriptomics
Citations
92 citations
Cites background from "RNA-Seq: a revolutionary tool for t..."
...As a consequence, RNA-seq allows for a large dynamic range of expression levels over which transcripts can be detected and has increased sensitivity for genes expressed at either very high or very low levels when compared to microarrays [36]....
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...An advantage of RNA-seq, when compared to microarrays, is that it is not limited to detecting transcripts that correspond to known genomic sequences, background signals are low and it does not have an upper limit for quantification [36]....
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92 citations
92 citations
Cites background from "RNA-Seq: a revolutionary tool for t..."
...Comparing with microarrays, RNA-Seq provides much richer information about the transcriptome, and also provides better resolution and higher accuracy when it is used for the conventional gene expression study [33]....
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92 citations
Cites methods from "RNA-Seq: a revolutionary tool for t..."
...Generally speaking, to exploit the dynamic information from RNA-seq data, a number of experimental and computational challenges need to be addressed, including the alignment of RNA-Seq reads, identification of mRNA isoforms, and estimation of mRNA isoform expression levels....
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...The identification of transcribed mRNA isoforms from the RNA-seq reads alignment and estimation of mRNA isoforms expression levels are basic problems to extract the dynamic information....
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...[87] Wang, K., Singh, D., Zeng, Z., Coleman, S. J. et al., MapSplice: accurate mapping of RNA-seq reads for splice junction discovery....
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...With the quick development of the next-generation sequencing technology, RNA-seq [84] is an increasingly popular method to study gene expression....
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...However, the mRNA isofrom expression levels can neither directly be obtained from RNA-seq data, nor be measured by the quantity of reads directly [88]....
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91 citations
Cites background from "RNA-Seq: a revolutionary tool for t..."
...RNA-Seq allows for sequencing and quantification of transcripts in a cell or tissue at unprecedented depth [77]....
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References
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"RNA-Seq: a revolutionary tool for t..." refers methods in this paper
...There are several programs for mapping reads to the genome, including ELAND, SOA...
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