RNA-Seq: a revolutionary tool for transcriptomics
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TLDR
The RNA-Seq approach to transcriptome profiling that uses deep-sequencing technologies provides a far more precise measurement of levels of transcripts and their isoforms than other methods.Abstract:
RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.read more
Citations
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RNA Sequencing and Analysis
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Improving genetic diagnosis in Mendelian disease with transcriptome sequencing
Beryl B. Cummings,Beryl B. Cummings,Jamie L. Marshall,Jamie L. Marshall,Taru Tukiainen,Taru Tukiainen,Monkol Lek,Sandra Donkervoort,A. Reghan Foley,Véronique Bolduc,Leigh B. Waddell,Leigh B. Waddell,Sarah A. Sandaradura,Sarah A. Sandaradura,Gina L. O’Grady,Gina L. O’Grady,Elicia Estrella,Hemakumar M. Reddy,Fengmei Zhao,Fengmei Zhao,Ben Weisburd,Ben Weisburd,Konrad J. Karczewski,Konrad J. Karczewski,Anne H. O’Donnell-Luria,Anne H. O’Donnell-Luria,Daniel P. Birnbaum,Daniel P. Birnbaum,Anna Sarkozy,Ying Hu,Hernan Gonorazky,Kristl G. Claeys,Himanshu Joshi,Adam Bournazos,Adam Bournazos,Emily C. Oates,Emily C. Oates,Roula Ghaoui,Roula Ghaoui,Mark R. Davis,Nigel G. Laing,Ana Töpf,Peter B. Kang,Peter B. Kang,Alan H. Beggs,Kathryn N. North,Volker Straub,James J. Dowling,Francesco Muntoni,Nigel F. Clarke,Nigel F. Clarke,Sandra T. Cooper,Sandra T. Cooper,Carsten G. Bönnemann,Daniel G. MacArthur,Daniel G. MacArthur +55 more
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Computational analysis of bacterial RNA-Seq data
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Data quality aware analysis of differential expression in RNA-seq with NOISeq R/Bioc package
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