RNA-Seq: a revolutionary tool for transcriptomics
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Cites background from "RNA-Seq: a revolutionary tool for t..."
...Comparing these two technologies, RNA-Seq is believed to have increased sensitivity, improved linearity, and a vastly larger dynamic range (Marioni et al., 2008; Mortazavi et al., 2008; Wang et al., 2009)....
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Cites background or methods from "RNA-Seq: a revolutionary tool for t..."
...RNA sequencing could be modeled as a random sampling process, in which each read is sampled independently and uniformly from every possible nucleotides in the sample (Jiang et al., 2009)....
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...2College of Computer Science and Technology, Jilin University, Changchun 130012, China....
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...Though it has been reported that sequencing platform has low background noise (Marioni et al., 2008; Wang et al., 2009), technical replicates would still be informative for quality control and to estimate the variation due to different machines or platforms....
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...Using such platforms to sequence cDNA samples (RNA-seq) has been shown as a powerful method to analyze the transcriptome of eukaryotic genomes (Wang et al., 2009)....
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"RNA-Seq: a revolutionary tool for t..." refers methods in this paper
...There are several programs for mapping reads to the genome, including ELAND, SOA...
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