RNA-Seq: a revolutionary tool for transcriptomics
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376 citations
Cites background from "RNA-Seq: a revolutionary tool for t..."
...While de novo assembly is mainly used to blindly reconstruct an unknown genome or transcriptome, read alignment has several purposes: when the original material is mRNA (RNAseq), it allows to precisely measure levels of transcripts and to identify splicing isoforms [8]....
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372 citations
370 citations
Cites methods from "RNA-Seq: a revolutionary tool for t..."
...RNA-Seq can reliably be used to correct gene annotations, confirm new and existing splice forms, analyse UTR regions, define non-coding RNAs or find new transcripts (Wang et al., 2009)....
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370 citations
Cites background from "RNA-Seq: a revolutionary tool for t..."
...…approaches, highthroughput sequencing technologies, referring to as RNASeq, have much greater power to distinguish between paralogous genes, detect low or high abundance transcripts, and allow replicate quantification based on the number of sequences obtained (Wang et al., 2009)....
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...Different from the traditional hybridization-based approaches, highthroughput sequencing technologies, referring to as RNASeq, have much greater power to distinguish between paralogous genes, detect low or high abundance transcripts, and allow replicate quantification based on the number of sequences obtained (Wang et al., 2009)....
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367 citations
References
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"RNA-Seq: a revolutionary tool for t..." refers methods in this paper
...There are several programs for mapping reads to the genome, including ELAND, SOA...
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