RNA-Seq: a revolutionary tool for transcriptomics
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Cites background from "RNA-Seq: a revolutionary tool for t..."
...The use of computational solutions to resolve reads into miRNAs suffers from the risk of reporting putative sequences that do not have real-world correlates (9)....
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Cites methods from "RNA-Seq: a revolutionary tool for t..."
...This approach has recently been used for transcriptome profiling in a method called RNA-seq that is expected to allow major breakthroughs in transcriptome analysis (Mortazavi et al. 2008; Nagalakshmi et al. 2008; Wilhelm et al 2008; Wang et al. 2009; Montgomery et al. 2010)....
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...major breakthroughs in transcriptome analysis (Mortazavi et al. 2008; Nagalakshmi et al. 2008; Wilhelm et al 2008; Wang et al. 2009; Montgomery et al. 2010)....
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356 citations
Cites background from "RNA-Seq: a revolutionary tool for t..."
...While transcriptomics and proteomics are increasingly more quantitative (i.e., RNA-seq in transcriptomics and stable labeled isotope tagging in proteomics), it is increasingly pertinent to compare the applicability and accuracy/precision of quantification strategies (e.g., absolute vs. relative quantification)....
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...Over the past decade technological advancements in next-generation DNA sequencing [13], SNP-chip profiling [14], transcriptome measurements via RNA-seq [15], SWATH-based proteomics [16], and metabolomics via UPLC-MS and GC-MS techniques [17,18] have greatly increased the ease, and significantly reduced the cost, of collecting rich, multi-omics data....
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"RNA-Seq: a revolutionary tool for t..." refers methods in this paper
...There are several programs for mapping reads to the genome, including ELAND, SOA...
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