RNA-Seq: a revolutionary tool for transcriptomics
Citations
227 citations
Cites background from "RNA-Seq: a revolutionary tool for t..."
...Moreover, because of the low redundancy of sequencing reads, EST data are not suitable to quantitatively estimate transcript abundance [16,18]....
[...]
...Besides the high throughput of RNAseq overcomes the shortcoming of low redundancy of sequencing reads in EST data, which makes EST data not suitable for quantitative measuring transcript abundance [16,18]....
[...]
226 citations
226 citations
225 citations
224 citations
Cites background or methods from "RNA-Seq: a revolutionary tool for t..."
...A recently developed alternative approach, called RNA-Seq, has the potential to overcome these difficulties [3]....
[...]
...To avoid UTR ambiguity in the annotation and boundary bias in the sequencing [3], we truncated all UTRs and a further 100 nucleotides on both ends....
[...]
References
12,293 citations
4,437 citations
2,927 citations
2,834 citations
2,729 citations
"RNA-Seq: a revolutionary tool for t..." refers methods in this paper
...There are several programs for mapping reads to the genome, including ELAND, SOA...
[...]