RNA-Seq: a revolutionary tool for transcriptomics
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207 citations
Cites methods from "RNA-Seq: a revolutionary tool for t..."
...RNA-sequencing (RNA-seq) is a recently developed approach that allows an unbiased examination of the transcriptome to be performed using high-throughput DNA sequencing [1-3]....
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207 citations
Cites background from "RNA-Seq: a revolutionary tool for t..."
...Biases have been found to be related to GC content of the sequence [54], the use of the random hexamer primers [55], 30 and 50 depletion or bias towards 30-end [36], and bias toward specific RNA species [56]....
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...If consistent results are obtained from biological replicates, the count of reads from a gene is more likely to reflect the expression level [36]....
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205 citations
204 citations
Cites background from "RNA-Seq: a revolutionary tool for t..."
...RNA-seq allows the sensitive detection of lowly expressing transcripts (Wang et al. 2009) and does not depend on current gene annotations....
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...Key words: long intergenic noncoding RNAs, modENCODE, transcriptional regulation, evolution, development....
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204 citations
References
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"RNA-Seq: a revolutionary tool for t..." refers methods in this paper
...There are several programs for mapping reads to the genome, including ELAND, SOA...
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