RNA-Seq: a revolutionary tool for transcriptomics
Citations
154 citations
Cites background from "RNA-Seq: a revolutionary tool for t..."
...RNA-Seq is a revolutionary technology for the accurate comparison of transcriptome profiles based on next-generation sequencing (Wang et al. 2009)....
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154 citations
Cites methods from "RNA-Seq: a revolutionary tool for t..."
...For this RNAseq approach either fragmented mRNA or fragmented cDNA [22] can be used as input and read lengths ranging from 100 nucleotides (nts), 250 nts and 500 nts modal length can be received depending on the sequencer and sequencing kit employed, GS 20, GS FLX Standard Series and GS FLX Titanium Series, respectively (reviewed in [21,23])....
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154 citations
Additional excerpts
...More recently, the advent of second-generation sequencing technologies, and the development of the RNA-seq method, has made it possible to sequence the entire transcriptome [10]....
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153 citations
153 citations
Cites background from "RNA-Seq: a revolutionary tool for t..."
...High-throughput transcriptome sequencing and digital gene expression (DGE) tag profiling are efficient and economic choice for characterizing non-model organisms without a reference genome [9,10]....
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References
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"RNA-Seq: a revolutionary tool for t..." refers methods in this paper
...There are several programs for mapping reads to the genome, including ELAND, SOA...
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