RNA-Seq: a revolutionary tool for transcriptomics
Citations
14,524 citations
Cites background or methods from "RNA-Seq: a revolutionary tool for t..."
...RNA-Seq is a powerful technology for analyzing transcriptomes that is predicted to replace microarrays [1]....
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...While RNASeq is generally accepted as being a more accurate quantification technology than microarrays [1], it 0 20 40 60 80 100 0 20 40 60 80 10 0...
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...In contrast with IsoEM, RSEM is capable of modeling non-uniform sequence-independent read start position distributions (RSPDs), such as 3’-biased distributions that are produced by some RNA-Seq protocols [1]....
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7,023 citations
Cites background from "RNA-Seq: a revolutionary tool for t..."
...For example, in gene-expression studies microarrays are now being replaced by seq-based methods , which can identify and quantify rare transcripts without prior knowledge of a particular gene and can provide information regarding alternative splicing and sequence variation in identified gene...
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6,369 citations
6,042 citations
Cites background from "RNA-Seq: a revolutionary tool for t..."
...It has been suggested that ‘One particularly powerful advantage of RNA-seq is that it can capture transcriptome dynamics across different tissues or conditions without sophisticated normalization of data sets’ [5]....
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5,980 citations
Cites methods from "RNA-Seq: a revolutionary tool for t..."
...High-throughput RNA sequencing (RNA-Seq) has emerged as a powerful method for transcriptomic analysis (1), widely used for understanding gene functions and biological patterns, finding candidate drug targets and identifying biomarkers for disease classification and diagnosis (2)....
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References
421 citations
"RNA-Seq: a revolutionary tool for t..." refers background in this paper
...Specialized microarrays have also been designed; for example, arrays with probes spanning exon junctions can be used to detect and quantify distinct spliced isoform...
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Additional excerpts
...In principle, any high-throughput sequencing technolog...
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