Journal ArticleDOI
Role of apoptosis and necrosis in cell death induced by nanoparticle-mediated photothermal therapy
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TLDR
It is found that the cell death response to photothermal therapy was dependent on the GNR localization, and it was demonstrated that nanorods targeted to the perinuclear region irradiated at 37.5 W/cm2 laser fluence rate led to maximum cell destruction with the ‘cleaner’ method of apoptosis, at similar percentages as other anti-cancer targeted therapies.Abstract:
Current cancer therapies can cause significant collateral damage due to a lack of specificity and sensitivity. Therefore, we explored the cell death pathway response to gold nanorod (GNR)-mediated photothermal therapy as a highly specific cancer therapeutic to understand the role of apoptosis and necrosis during intense localized heating. By developing this, we can optimize photothermal therapy to induce a maximum of ‘clean’ cell death pathways, namely apoptosis, thereby reducing external damage. GNRs were targeted to several subcellular localizations within colorectal tumor cells in vitro, and the cell death pathways were quantitatively analyzed after photothermal therapy using flow cytometry. In this study, we found that the cell death response to photothermal therapy was dependent on the GNR localization. Furthermore, we demonstrated that nanorods targeted to the perinuclear region irradiated at 37.5 W/cm2 laser fluence rate led to maximum cell destruction with the ‘cleaner’ method of apoptosis, at similar percentages as other anti-cancer targeted therapies. We believe that this indicates the therapeutic potential for GNR-mediated photothermal therapy to treat cancer effectively without causing damage to surrounding tissue.read more
Citations
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Journal ArticleDOI
Recent Progress in Cancer Thermal Therapy Using Gold Nanoparticles
TL;DR: This work focuses on the developments and progress in nanoparticle design for photothermal cancer therapy since 2010, which includes in vitro and in vivo studies and the recent progression of gold nanoparticle photothermal therapy toward clinical cancer treatment.
Journal ArticleDOI
Efficacy, long-term toxicity, and mechanistic studies of gold nanorods photothermal therapy of cancer in xenograft mice.
Moustafa R. K. Ali,Mohammad Aminur Rahman,Yue Wu,Tiegang Han,Xianghong Peng,Megan A. Mackey,Dongsheng Wang,Hyung Ju C. Shin,Zhuo Georgia Chen,Haopeng Xiao,Ronghu Wu,Yan Tang,Dong M. Shin,Mostafa A. El-Sayed,Mostafa A. El-Sayed +14 more
TL;DR: The optimized properties of AuNRs and the conditions of PPTT to achieve maximal induction of tumor apoptosis and quantitative proteomics analysis in mouse tumor tissues suggest that the Au NRs-PPTT has potential as an approach to cancer therapy, and a long-term toxicity study shows that the platform is effective and safe for cancer therapy in mouse models.
Journal ArticleDOI
Polydopamine-coated nucleic acid nanogel for siRNA-mediated low-temperature photothermal therapy.
Fei Ding,Xihui Gao,Xiangang Huang,Huan Ge,Miao Xie,Jiwen Qian,Jie Song,Yuehua Li,Xinyuan Zhu,Chuan Zhang +9 more
TL;DR: A polydopamine (PDA)-coated nucleic acid nanogel as a therapeutic complex for siRNA-mediated low-temperature PTT shows the capability of effective ablating the tumor under relatively mild condition.
Journal ArticleDOI
Photothermal Therapy Generates a Thermal Window of Immunogenic Cell Death in Neuroblastoma.
TL;DR: A tunable immune response to heat generated by PBNP-PTT, which should be critically engaged in the administration of PTT for maximizing its therapeutic benefits, is demonstrated.
Journal ArticleDOI
Progress and future of in vitro models to study translocation of nanoparticles.
Hedwig M. Braakhuis,Samantha K. Kloet,Sanja Kezic,Frieke Kuper,Margriet V. D. Z. Park,Susann Bellmann,Meike van der Zande,Séverine Le Gac,Petra Krystek,Ruud J. B. Peters,Ivonne M.C.M. Rietjens,Hans Bouwmeester +11 more
TL;DR: To further develop in vitro models to be used in risk assessment, the current strategy to improve the models to more closely mimic the human situation by using co-cultures of different cell types and microfluidic approaches to better control the tissue microenvironments are essential.
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Journal ArticleDOI
Cancer Cell Imaging and Photothermal Therapy in the Near-Infrared Region by Using Gold Nanorods
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