Routine In Vitro Culture of Plasmodium falciparum: Experimental Consequences?
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Cites methods from "Routine In Vitro Culture of Plasmod..."
...REAGENT or RESOURCE SOURCE IDENTIFIER Peptide substrate GRTGRRNSI-NH2 Sigma-Aldrich Cat# SCP0212 Renilla-Glo(R) Luciferase Assay System Promega Cat# E2750 QiAmp DNA Blood Mini kit Qiagen https://www.qiagen.com/ Deposited Data PKG crystal structure (Baker et al., 2017) PDB ID: 5DYK Experimental Models: Organisms/Strains Anopheles stephensi mosquitoes Insectary Core Facility, New York University N/A Plasmodium falciparum asexual blood stage parasites Goldberg lab, Washington University 3D7-A10 clone P. falciparum asexual blood stage parasites Fidock lab, Columbia UniversityMedical Center Dd2-B2 clone P. falciparum NF54 CDPK1 T145M line Miller lab, Malaria Cell Biology, NIAID, NIH (Bansal et al., 2018) CDPK1T145M P. falciparum 3D7elo1-pfs16-CBG99 transgenic parasites Fidock lab, Columbia UniversityMedical Center (Cevenini et al., 2014) N/A BALB/c female mice The Jackson Laboratory https://www.jax.org/ P. berghei ANKA (Pb-Luc) Insectary Core Facility, New York University Pb-Luc, also known as P. bergheiANKA-GFP-Luc-SMCON Oligonucleotides All primers and cloning fragments are shown in Table S1 (J) and (K) N/A Recombinant DNA pSN054 vector (Nasamu et al., 2019) N/A pET28-PfTKL3-SAM(V54E)-KD (Abdi et al., 2010) N/A Software and Algorithms GraphPad Prism Version 8 GraphPad Software, San Diego, CA, USA www.graphpad.com ICY BioImage Analysis (Delves et al., 2016) http://icy.bioimageanalysis.org/ R Studio RStudio Team, 2015 http://www.rstudio.com/ Proteome Discoverer Thermo Scientific https://www.thermofisher.com/us/en/ home.html Mascot Matrix Science http://www.matrixscience.com/ Scaffold Q+S Proteome Software http://www.proteomesoftware.com/ ChemiDoc MP System and Image Lab 5.2.0 Bio-Rad https://www.bio-rad.com/ El- MAVEN software (Agrawal et al., 2019) https://elucidatainc.github.io/ElMaven/ Schrodinger molecular modeling suite Schrodinger http://www.schrodinger.com/ ll OPEN ACCESS Article...
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...Dual Gamete Formation Assay Pf NF54 gametocytes were generated as reported elsewhere (Delves et al., 2016)....
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...…et al., 2010) N/A Software and Algorithms GraphPad Prism Version 8 GraphPad Software, San Diego, CA, USA www.graphpad.com ICY BioImage Analysis (Delves et al., 2016) http://icy.bioimageanalysis.org/ R Studio RStudio Team, 2015 http://www.rstudio.com/ Proteome Discoverer Thermo Scientific…...
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...Both time lapse images of exflagellation and fluorescence images of female gametes were automatically quantified using custom scripts in ICY BioImage Analysis (Delves et al., 2016)....
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...Pf NF54 gametocytes were generated as reported elsewhere (Delves et al., 2016)....
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38 citations
27 citations
Cites methods from "Routine In Vitro Culture of Plasmod..."
...…used method to enhance sexual conversion and obtain large numbers of gametocytes in vitro relies on overgrowing blood-stage cultures (the ‘crash method’) (Delves et al., 2016) and/or maintaining the cultures with parasite-conditioned (spent) medium (Brancucci et al., 2015; Fivelman et al., 2007)....
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...The most commonly used method to enhance sexual conversion and obtain large numbers of gametocytes in vitro relies on overgrowing blood-stage cultures (the ‘crash method’) (Delves et al., 2016) and/or maintaining the cultures with parasite-conditioned (spent) medium (Brancucci et al....
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23 citations
22 citations
Cites background from "Routine In Vitro Culture of Plasmod..."
...ticularly in earlier trophic stages of the intra-erythrocytic cycle [47], and although we did not consider oxygen me-...
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