Sample-pooling strategy for SARS-CoV-2 detection among students and staff of the university of Sannio
Immacolata Polvere,Elena Silvestri,Lina Sabatino,Antonia Giacco,Stefania Iervolino,Teresa Peluso,Rosa Guida,Lucrezia Zerillo,Romualdo Varricchio,Silvia D’Andrea,Serena Voccola,Jessica Raffaella Madera,Alberto Zullo,Romania Stilo,Pasquale Vito,Tiziana Zotti +15 more
- Vol. 11, Iss: 7, pp 1166
Reads0
Chats0
TLDR
In this paper, a simple monodimensional sample pooling strategy was used to detect SARS-CoV-2 positive cases in a community of asymptomatic individuals with minor requirements of reagents and time when compared to normal testing procedures.Abstract:
Since the beginning of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pandemic, it has been clear that testing large groups of the population was the key to stem infection and prevent the effects of the coronavirus disease of 2019, mostly among sensitive patients. On the other hand, time and cost-sustainability of virus detection by molecular analysis such as reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) may be a major issue if testing is extended to large communities, mainly asymptomatic large communities. In this context, sample-pooling and test grouping could offer an effective solution. Here we report the screening on 1195 oral-nasopharyngeal swabs collected from students and staff of the Universita degli Studi del Sannio (University of Sannio, Benevento, Campania, Italy) and analyzed by an in-house developed multiplex RT-qPCR for SARS-CoV-2 detection through a simple monodimensional sample pooling strategy. Overall, 400 distinct pools were generated and, within 24 h after swab collection, five positive samples were identified. Out of them, four were confirmed by using a commercially available kit suitable for in vitro diagnostic use (IVD). High accuracy, sensitivity and specificity were also determined by comparing our results with a reference IVD assay for all deconvoluted samples. Overall, we conducted 463 analyses instead of 1195, reducing testing resources by more than 60% without lengthening diagnosis time and without significant losses in sensitivity, suggesting that our strategy was successful in recognizing positive cases in a community of asymptomatic individuals with minor requirements of reagents and time when compared to normal testing procedures.read more
Citations
More filters
Journal ArticleDOI
Evaluation of FAST COVID-19 SARS-CoV-2 Antigen Rapid Test Kit for Detection of SARS-CoV-2 in Respiratory Samples from Mildly Symptomatic or Asymptomatic Patients
Immacolata Polvere,Serena Voccola,Silvia D'Andrea,Lucrezia Zerillo,Romualdo Varricchio,Jessica Raffaella Madera,Romania Stilo,Pasquale Vito,Tiziana Zotti +8 more
TL;DR: Considering short lead times, low cost, and opportunities for decentralized testing, the Ag-RDT test can enhance the efforts to control SARS-CoV-2 spread in several settings.
Journal ArticleDOI
Prospective evaluation of specimen pooling strategy for detection of SARS-CoV-2 using pools of five and six specimens.
Georgios Meletis,Styliani Pappa,Maria Exindari,Georgia Gioula,Evangelia Giosi,Antonios Katsoulas,Ilias Mavrovouniotis,Anna Papa +7 more
TL;DR: In this paper, a prospective evaluation of the pooling strategy was performed using known positive specimens, previously tested individually, and their respective pools of plus four (5X), five (6X) and nine (10X) known negative specimens.
References
More filters
Journal ArticleDOI
Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China
Chaolin Huang,Yeming Wang,Xingwang Li,Lili Ren,Jianping Zhao,Yi Hu,Li Zhang,Guohui Fan,Jiuyang Xu,Xiaoying Gu,Zhenshun Cheng,Ting Yu,Jia'an Xia,Yuan Wei,Wenjuan Wu,Xuelei Xie,Wen Yin,Li Hui,Min Liu,Yan Xiao,Hong Gao,Li Guo,Jungang Xie,Guang-Fa Wang,Rongmeng Jiang,Zhancheng Gao,Qi Jin,Jianwei Wang,Bin Cao +28 more
TL;DR: The epidemiological, clinical, laboratory, and radiological characteristics and treatment and clinical outcomes of patients with laboratory-confirmed 2019-nCoV infection in Wuhan, China, were reported.
Journal ArticleDOI
A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster.
Jasper Fuk-Woo Chan,Shuofeng Yuan,Kin-Hang Kok,Kelvin K. W. To,Kelvin K. W. To,Hin Chu,Jin Yang,Fanfan Xing,Jieling Liu,Cyril C. Y. Yip,Rosana W.S. Poon,Hoi Wah Tsoi,Simon Kam Fai Lo,Kwok-Hung Chan,Vincent Kwok-Man Poon,Wan Mui Chan,Jonathan Daniel Ip,Jian Piao Cai,Vincent C.C. Cheng,Honglin Chen,Honglin Chen,Christopher K.M. Hui,Kwok-Yung Yuen +22 more
TL;DR: The findings are consistent with person-to-person transmission of this novel coronavirus in hospital and family settings, and the reports of infected travellers in other geographical regions.
Journal ArticleDOI
Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR.
Victor M. Corman,Olfert Landt,Marco Kaiser,Richard Molenkamp,Adam Meijer,Daniel K.W. Chu,Tobias Bleicker,Sebastian Brünink,Julia Schneider,Marie Luisa Schmidt,Daphne G.J.C. Mulders,Bart L. Haagmans,Bas van der Veer,Sharon van den Brink,Lisa Wijsman,Gabriel Goderski,Jean Louis Romette,Joanna Ellis,Maria Zambon,Malik Peiris,Herman Goossens,Chantal B.E.M. Reusken,Marion Koopmans,Christian Drosten +23 more
TL;DR: A validated diagnostic workflow for 2019-nCoV is presented, its design relying on close genetic relatedness of 2019- nCoV with SARS coronavirus, making use of synthetic nucleic acid technology.
Journal ArticleDOI
A novel coronavirus outbreak of global health concern.
Journal ArticleDOI
Laboratory Diagnosis of COVID-19: Current Issues and Challenges.
TL;DR: In the preanalytical stage, collecting the proper respiratory tract specimen at the right time from the right anatomic site is essential for a prompt and accurate molecular diagnosis of COVID-19, and real-time reverse transcription-PCR assays remain the molecular test of choice for the etiologic diagnosis of SARS-CoV-2 infection while antibody-based techniques are being introduced as supplemental tools.
Related Papers (5)
Pooled testing for COVID-19 diagnosis by real-time RT-PCR: A multi-site comparative evaluation of 5- & 10-sample pooling
Ira Praharaj,Amita Jain,Mini P Singh,Anukumar Balakrishnan,Rahul Dhodapkar,Biswajyoti Borkakoty,Munivenkatappa Ashok,Pradeep Das,Debasis Biswas,Usha Kalawat,Jyotirmayee Turuk,A P Sugunan,Shantanu Prakash,Anirudh K. Singh,Rajamani Barathidasan,Subhra Subhadra,Jyotsnamayee Sabat,M J Manjunath,Poonam Kanta,Nagaraja Mudhigeti,Rahul Hazarika,Hricha Mishra,Kumar Abhishek,C Santhalembi,Manas Ranjan Dikhit,Neetu Vijay,Jitendra Narayan,Harmanmeet Kaur,Sidhartha Giri,Nivedita Gupta +29 more