Journal ArticleDOI
Screening lambdagt recombinant clones by hybridization to single plaques in situ
WD Benton,Ronald W. Davis +1 more
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A rapid, direct method for screening single plaques of Agt recombinant phage is described, which allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.Abstract:
A rapid, direct method for screening single plaques of Agt recombinant phage is described. The method allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.read more
Citations
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A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: In this article, a technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, where DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers.
Journal ArticleDOI
Positional cloning of the mouse obese gene and its human homologue
Yiying Zhang,Ricardo Proenca,Ricardo Proenca,Margherita Maffei,Marisa Barone,Marisa Barone,Lori Leopold,Lori Leopold,Jeffrey M. Friedman,Jeffrey M. Friedman +9 more
TL;DR: The ob gene product may function as part of a signalling pathway from adipose tissue that acts to regulate the size of the body fat depot.
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A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
Robert S. Sikorski,Philip Hieter +1 more
TL;DR: A series of yeast shuttle vectors and host strains has been created to allow more efficient manipulation of DNA in Saccharomyces cerevisiae to perform most standard DNA manipulations in the same plasmid that is introduced into yeast.
References
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Journal ArticleDOI
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
TL;DR: This method can be used to isolate clones of ColE1 hybrid plasmids that contain Drosophila melanogaster genes for 18 and 28S rRNAs and any gene whose base sequence is represented in an available RNA.
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A colony bank containing synthetic CoI EI hybrid plasmids representative of the entire E. coli genome
Louise Clarke,John Carbon +1 more
TL;DR: Over 80 hybrid Col El-DNA (E. coli), plasmid-bearing clones have been identified in the colony bank, and about 40 known E. coli genes have been tentatively assigned to these various plasmids.
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Hybridization of RNA to double-stranded DNA: formation of R-loops
TL;DR: The R-loop formation is maximal at the temperature at which half of the duplex DNA is irreversibly converted to single-stranded DNA and falls precipitously a few degrees above or below that temperature and the rate is proportional to the RNA concentration.
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Functional genetic expression of eukaryotic DNA in Escherichia coli
TL;DR: From genetic experiments, it is concluded that expression of the segment of yeast DNA results in the production of a diffusible substance and that transcription necessary for the complementation is most likely initiated from the segments of eukaryotic DNA.
Journal ArticleDOI
Analysis of chromosomal integration and deletions of yeast plasmids
TL;DR: In this article, three different plasmid DNAs from six strains of Saccharomyces cerevisiae were compared and three different groups of plasmids were found, designated Scp 1, Scp 2 and Scp 3, with monomer lengths of 6.19, 6.06 and 5.97 kilobases.