Book ChapterDOI
SDS Polyacrylamide Gel Electrophoresis of Proteins.
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Probably the most widely used of techniques for analyzing mixtures of proteins is SDS polyacrylamide gel electrophoresis, where proteins are reacted with the anionic detergent, sodium dodecylsulfate (SDS, or sodium lauryl sulfate) to form negatively charged complexes.Abstract:
Probably the most widely used of techniques for analyzing mixtures of proteins is SDS polyacrylamide gel electrophoresis. In this technique, proteins are reacted with the anionic detergent, sodium dodecylsulfate (SDS, or sodium lauryl sulfate) to form negatively charged complexes. The amount of SDS bound by a protein, and so the charge on the complex, is roughly proportional to its size. Commonly, about 1.4 g SDS is bound per 1 g protein, although there are exceptions to this rule. The proteins are generally denatured and solubilized by their binding of SDS, and the complex forms a prolate elipsoid or rod of a length roughly proportionate to the protein's molecular weight. Thus, proteins of either acidic or basic pI form negatively charged complexes that can be separated on the bases of differences in charges and sizes by electrophoresis through a sieve-like matr ix of polyacrylamide gel.read more
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Purification to homogeneity and characterization of a novel Pseudomonas putida chromate reductase.
TL;DR: X-ray absorption near-edge-structure spectra showed quantitative conversion of chromate to Cr(III) during the enzyme reaction, and bioremediation can be effective in removing chromate from the environment, especially if the bacterial propensity for such removal is enhanced by genetic and biochemical engineering.
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Chromate-Reducing Properties of Soluble Flavoproteins from Pseudomonas putida and Escherichia coli
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An overview of technical considerations for Western blotting applications to physiological research.
Joseph J. Bass,Daniel J. Wilkinson,Debbie Rankin,Bethan E. Phillips,Nathaniel J. Szewczyk,Kenneth Smith,Philip J. Atherton +6 more
TL;DR: The present review aims to provide a detailed description and critique of WB procedures and technicalities, from sample collection through preparation, blotting and detection, to analysis of the data collected, to produce reproducible and reliable blots.
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References
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Journal ArticleDOI
Analysis of bacteriophage T7 early RNAs and proteins on slab gels
TL;DR: The RNAs and proteins specified by five early genes of bacteriophage T7 have been identified by electrophoresis on sodium dodecyl sulfate, polyacrylamide gels using a slab gel system in which 25 samples can be run simultaneously and then dried for autoradiography.