Open AccessDOI
Seed Paternity Analysis Using SSR Markers to Assess Successful Pollen Donors in Mixed Olive Orchards
Gabriela Vuletin Selak,Alenka Baruca Arbeiter,Julián Cuevas,Slavko Perica,Petar Pujic,Marina Raboteg Božiković,Dunja Bandelj +6 more
- Vol. 10, Iss: 11, pp 2356
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TLDR
In this paper, the paternity analysis of olive embryos was performed using simple sequence repeats (SSRs), microsatellite markers used for the identification of potential pollen donors of a mixed olive orchard during two consecutive years.Abstract:
The olive tree (Olea europaea L.) is a wind-pollinated crop that exhibits an extreme alternate bearing habit. To improve fruit set, several methods have been used to determine the most successful compatible combinations of cultivars. In this study, priority is given to seed paternity analysis based on simple sequence repeats (SSRs), microsatellite markers used for the identification of potential pollen donors of cultivar ‘Oblica’ in a mixed olive orchard during two consecutive years. Seven microsatellite primers were successfully used to examine the paternity of olive embryos from ‘Oblica’ mother trees. Embryos were considered as a product of self-fertilization if only maternal alleles were present, but not a single case of self-fertilization was found among all the embryos analyzed. Two dominant pollen donors were not the closest nor the cultivars with the highest number of trees in the orchard, suggesting that cross-compatibility may have a key role in determining pollen donor success. In our earlier studies, pollen tube growth and fertilization success correlated with fruit set when controlled crosses between cultivars were performed; however, some discrepancy might appear compared to paternity analyses when mother trees have a free choice among different pollen sources from cultivars growing in their surroundings.read more
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Journal ArticleDOI
Determining Pollinizer Success Rates among Several Apple (Malus domestica L.) Cultivars Using Microsatellite Markers
Fuad Gaši,Naris Pojskić,Belma Kalamujić Stroil,Oddmund Frøynes,Milica Fotirić Akšić,Mekjell Meland +5 more
TL;DR: In order to determine the pollinizer success rates between twelve apple cultivars in 2019 and 2022, 671 apple embryos were collected from 19 different orchards in Ullensvang (southwestern Norway) and Svelvik (southeastern Norway) as discussed by the authors .
Journal ArticleDOI
The effective pollination period of the olive cultivar ‘Arbequina’ (Olea europaea L.) in a non-traditional region
TL;DR: In this paper , the authors determined the EPP for the main olive cultivar in Uruguay by two methodologies: sequential pollination of flowers of different ages, and analysis of its components (stigmatic receptivity, ovule viability, and pollen tube growth).
References
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Journal ArticleDOI
Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment.
TL;DR: This paper showed that the likelihood equations used by versions 1.0 and 2.0 of CERVUS to accommodate genotyping error miscalculate the probability of observing an erroneous genotype.
Journal ArticleDOI
An economic method for the fluorescent labeling of PCR fragments
TL;DR: A poor man's approach to genotyping for research and high-throughput diagnostics and how it can be applied to medicine and science.
Journal ArticleDOI
Microsatellite analysis of population structure in Canadian polar bears.
TL;DR: Using eight hypervariable microsatellite loci to study the genetic relationships between four Canadian polar bear populations indicates that gene flow between local populations is restricted despite the long‐distance seasonal movements undertaken by polar bears.
Journal ArticleDOI
Comparisons of three probability formulae for parentage exclusion
A Jamieson,St. C S Taylor +1 more
TL;DR: Three general formulae calibrate the average capability of marker systems to dispute falsely reported pedigree records in uniparous species and facilitate the calculation of maximal average exclusion values over a range of hypothetical markers.