Journal ArticleDOI
Selection of large diversities of antiidiotypic antibody fragments by phage display.
Steffen Goletz,Peter Christensen,Peter Kristensen,Dietmar Blohm,Ian M. Tomlinson,Greg Winter,Uwe Karsten +6 more
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TLDR
In this article, the authors describe a fast and reliable technique for generating large diversities of anti-iotypic single chain antibody fragments from non-immunized phagemid libraries using phage display.About:
This article is published in Journal of Molecular Biology.The article was published on 2002-02-01. It has received 140 citations till now. The article focuses on the topics: Phage display & Phagemid.read more
Citations
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Journal ArticleDOI
Active targeting schemes for nanoparticle systems in cancer therapeutics.
TL;DR: The targeting schemes explored for many of the reported nanoparticle systems suggest the great potential of targeted delivery to revolutionize cancer treatment.
Journal ArticleDOI
Nanoparticle Probes for the Detection of Cancer Biomarkers, Cells, and Tissues by Fluorescence
Alyssa B. Chinen,Chenxia M. Guan,Jennifer R. Ferrer,Stacey N. Barnaby,Timothy J. Merkel,Chad A. Mirkin +5 more
TL;DR: Overcoming Limitations in Nanoparticle Drug Delivery: Triggered, Intravascular Release to Improve Drug Penetration into Tumors and Design Considerations for Tumour-Targeted Nanoparticles.
Journal ArticleDOI
Applications of single-chain variable fragment antibodies in therapeutics and diagnostics.
TL;DR: This review will show the tremendous versatility and importance of scFv fragments as they provide the basic antigen binding unit for a multitude of engineered Abs for use as human therapeutics and diagnostics.
Journal ArticleDOI
Application of phage display to high throughput antibody generation and characterization
Darren Schofield,Anthony Richard Pope,Veronica Clementel,Jenny Buckell,Susan D.J. Chapple,Kay Clarke,Jennie S. Conquer,Anna M. Crofts,Sandra R.E. Crowther,Michael R. Dyson,Gillian Flack,Gareth J. Griffin,Yvette Hooks,William J. Howat,Anja Kolb-Kokocinski,Susan Kunze,Cecile D. Martin,Gareth Maslen,Joanne N. Mitchell,Maureen O'Sullivan,Rajika L. Perera,Wendy Roake,S. Paul Shadbolt,Karen Vincent,Anthony Warford,Wendy E. Wilson,Jane Xie,Joyce L. Young,John McCafferty +28 more
TL;DR: The potential of and the issues associated with genome-wide monoclonal antibody generation are demonstrated and illuminated by a high quality phage display library containing over 1010 human antibodies.
References
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Journal ArticleDOI
Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface
TL;DR: Foreign DNA fragments can be inserted into filamentous phage gene III to create a fusion protein with the foreign sequence in the middle that is incorporated into the virion, which retains infectivity and displays the foreign amino acids in immunologically accessible form.
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Phage antibodies: filamentous phage displaying antibody variable domains
TL;DR: It is shown that complete antibody V domains can be displayed on the surface of fd bacteriophage, that the phage bind specifically to antigen and that rare phage can be isolated after affinity chromatography.
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By-passing immunization: Human antibodies from V-gene libraries displayed on phage
James D. Marks,Hennie R. Hoogenboom,Timothy Peter Bonnert,John McCafferty,Andrew D. Griffiths,Greg Winter +5 more
TL;DR: The results suggest that a single large phage display library can be used to isolate human antibodies against any antigen, by-passing both hybridoma technology and immunization.
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Making Antibodies by Phage Display Technology
TL;DR: Human antibody fragments with many different binding specificities have been isolated from the same phage repertoire, including haptens, carbohydrates, secreted and cell surface proteins, viral coat proteins, and intracellular antigens from the lumen of the endoplasmic reticulum and the nucleus.