Selective and easy detection of microcystin-LR in freshwater using a bioactivated sensor based on multiwalled carbon nanotubes on filter paper.
TL;DR: The microcystin-LR (MC-LR) is a cyanobacterial toxin produced as a result of eutrophication in polluted water in warm weather conditions as mentioned in this paper.
About: This article is published in Biosensors and Bioelectronics.The article was published on 2021-07-24. It has received 3 citations till now.
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TL;DR: In this paper , a magnetic self-assembled covalent organic framework (COF) was synthesized via a facile approach by using a one-pot reaction of 1,6-bis (4-formylphenyl)-3,8-bis(4-aminophenyl)pyrene (BFBAPy).
4 citations
01 May 2023
TL;DR: In this article , the authors presented sensitive, highly rapid electrochemical measurement of airborne coronaviruses through condensation-based direct impaction onto carbon nanotube-coated porous paper working electrodes (PWEs).
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TL;DR: A review of the typical characteristics and binding mechanisms of various biorecognition elements, and how they relate to biosensor performance characteristics, specifically sensitivity, selectivity, reproducibility, and reusability are reviewed.
216 citations
TL;DR: Aptamers are short single-stranded RNA or DNA oligonucleotides capable of folding into complex 3D structures, enabling them to bind to a large variety of targets ranging from small ions to an entire organism, and they are superior regarding a longer shelf life, simple production and chemical modification.
Abstract: Soon after they were first described in 1990, aptamers were largely recognized as a new class of biological ligands that can rival antibodies in various analytical, diagnostic, and therapeutic applications. Aptamers are short single-stranded RNA or DNA oligonucleotides capable of folding into complex 3D structures, enabling them to bind to a large variety of targets ranging from small ions to an entire organism. Their high binding specificity and affinity make them comparable to antibodies, but they are superior regarding a longer shelf life, simple production and chemical modification, in addition to low toxicity and immunogenicity. In the past three decades, aptamers have been used in a plethora of therapeutics and drug delivery systems that involve innovative delivery mechanisms and carrying various types of drug cargos. However, the successful translation of aptamer research from bench to bedside has been challenged by several limitations that slow down the realization of promising aptamer applications as therapeutics at the clinical level. The main limitations include the susceptibility to degradation by nucleases, fast renal clearance, low thermal stability, and the limited functional group diversity. The solution to overcome such limitations lies in the chemistry of aptamers. The current review will focus on the recent arts of aptamer chemistry that have been evolved to refine the pharmacological properties of aptamers. Moreover, this review will analyze the advantages and disadvantages of such chemical modifications and how they impact the pharmacological properties of aptamers. Finally, this review will summarize the conjugation strategies of aptamers to nanocarriers for developing targeted drug delivery systems.
179 citations
TL;DR: In this paper, a two-step analytical electrical conductivity method is adopted to calculate the effective electrical conductivities of the carbon fiber (CF)-carbon nanotube (CNT)-polymer hybrid composite.
Abstract: A two-step analytical electrical conductivity method is adopted to calculate the effective electrical conductivity of the carbon fiber (CF)-carbon nanotube (CNT)-polymer hybrid composite. First, CNTs are dispersed into the non-conducting polymer matrix and the electrical conductivity of the CNT-polymer composite is obtained. Then, CFs are randomly distributed in the CNT-polymer composite and the effective electrical conductivity of CF-CNT-polymer hybrid composite is estimated. The effect of critical parameters, including the volume fraction, alignment, agglomerated state and aspect ratio of the CNTs and the potential barrier height of the polymer on the hybrid composite electrical conductivity is evaluated. Also, the influence of the content and aspect ratio of CFs on the electric conductive behavior of the polymer hybrid composites is investigated. The results show that the polymer hybrid composite with larger aspect ratio and off alignment of CNTs presents a higher electrical conductivity.
105 citations
96 citations
TL;DR: A novel biosensor is presented to address the lack of a reliable point-of-care drug monitoring system in the market that incorporates high selectivity and sensitivity by integrating aptamers as the recognition element and field-effect transistors as the signal transducer.
Abstract: During medical treatment it is critical to maintain the circulatory concentration of drugs within their therapeutic range. A novel biosensor is presented in this work to address the lack of a reliable point-of-care drug monitoring system in the market. The biosensor incorporates high selectivity and sensitivity by integrating aptamers as the recognition element and field-effect transistors as the signal transducer. The drug tenofovir was used as a model small molecule. The biointerface of the sensor is a binary self-assembled monolayer of specific thiolated aptamer and 6-mercapto-1-hexanol (MCH), whose ratio was optimized by electrochemical impedance spectroscopy measurements to enhance the sensitivity towards the specific target. Surface plasmon resonance, performed under different buffer conditions, shows optimum specific and little non-specific binding in phosphate buffered saline. The dose-response behavior of the field-effect biosensor presents a linear range between 1 nM and 100 nM of tenofovir and a limit of detection of 1.2 nM. Two non-specific drugs and one non-specific aptamer, tested as stringent control candidates, caused negligible responses. The applications were successfully extended to the detection of the drug in human serum. As demonstrated by impedance measurements, the aptamer-based sensors can be used for real-time drug monitoring.
67 citations