Journal ArticleDOI
Selenium-mediated differential response of beta-glucosidase and beta-galactosidase of germinating Trigonella foenum-graecum.
M. Sreekala,K. Lalitha +1 more
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TLDR
The study reveals a differential response to Se among the β-galactosidase and β-glucosid enzyme of T. foenumgraecum with increase in the levels of β-GalactOSidase activity.Abstract:
Beta-glucosidase and beta-galactosidase activity profile tested in different seeds during 24 h germination revealed reasonably high levels of activity in Vigna radiata, Cicer arietinum, and Trigonella foenum-graecum. In all seeds tested, beta-galactosidase activity was, in general, higher than that of beta-glucosidase. T. foenum-graecum seedlings exhibited maximal total and specific activities for both the enzymes during 72 h germination. Se supplementation as Na2SeO3 up to 0.75 ppm was found to be beneficial to growth and revealed selective enhancement of beta-galactosidase activity by 40% at 0.5 ppm Se. The activities of both the enzymes drastically decreased at 1.0 ppm level of Se supplementation. On the contrary, addition of Na2SeO3 in vitro up to 1 ppm to the enzyme extracts did not influence these activities. Hydrolytic rates of beta-glucosidase in both control and Se-supplemented groups were enhanced by 20% with 0.05 M glycerol in the medium and 30% at 0.1 M glycerol. The rates were marginally higher in Se-supplemented seedlings than the controls, irrespective of added glycerol in the medium. In contrast, hydrolysis by beta-galactosidase showed a trend of decrease in Se-supplemented seedlings compared to the control, when glycerol was present in the medium. Addition of Se in vitro in the assay medium showed no difference in the hydrolytic rate by beta-galactosidase when compared to control, while the activity of beta-glucosidase declined by 50%. Se-grown seedlings showed an enhancement of transglucosidation rate by 40% in the presence of 0.1 M glycerol. The study reveals a differential response to Se among the beta-galactosidase and beta-glucosidase of T. foenum-graecum with increase in the levels of beta-galactosidase activity.read more
Citations
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Journal ArticleDOI
Chromatographic speciation of anionic and neutral selenium compounds in Se-accumulating Brassica juncea (Indian mustard) and in selenized yeast.
TL;DR: In this article, the complementary role of selenium and sulfur specific chromatographic detection by HPLC with interfaced inductively coupled plasma mass spectrometry (ICP-MS) detection and by derivatization GC with interfacing atomic spectral emission was investigated.
Journal ArticleDOI
Selenium and salt interactions in sage (Salvia officinalis L.): Growth and yield, chemical content, ion uptake
Gulsum Yaldiz,Mahmut Camlica +1 more
TL;DR: In this article, the effect of se fertilization on sage (Salvia officinalis L.) under different salt stresses was examined and the results showed that Se treatments caused significant improvements in the dry weight (DW) of sage under salt stress.
Journal ArticleDOI
Physiological and toxic effects of selenium on seed germination of cowpea seedlings
Allan de Marcos Lapaz,Luiz Felipe de Melo Santos,Camila Hatsu Pereira Yoshida,Reges Heinrichs,Marcelo Campos,André Rodrigues dos Reis +5 more
TL;DR: In this article, the authors evaluated 11 concentrations of Se (0, 0.1; 0.5; 1, 5, 10, 20, 40, 80, 400, 800 mg.L-1) to determine the low and high (toxicity) critical levels to seed germination of cowpea (Vigna unguiculata).
Journal ArticleDOI
Seed Germination Response of Rapid-Cycling Brassica oleracea Grown Under Increasing Sodium Selenate
TL;DR: Observations provide evidence that Se accumulation in seeds is not likely to hamper breeding efforts for Se enrichment in B. oleracea at Se levels similar to this study.
Journal ArticleDOI
Efficiency of selenium compounds in breaking dormancy of Townsville stylo seeds
TL;DR: Although ethylene was required for dormancy breakage the compounds triggering the highest ethylene productions were not necessarily the same ones leading to the largest Ief(s), Hence it was concluded that the index is an overall integrating parameter, encompassing uptake of the compound, arrival to target cells and also post-ethylene biosynthesis effects.
References
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Journal ArticleDOI
Regulation of Selenoproteins
Raymond F. Burk,Kristina E. Hill +1 more
TL;DR: The selenoproteins most sensitive to selenium deficiency is liver cGSH-Px, and decreased synthesis of it under deficiency conditions might serve to increase theselenium available for synthesis of seleniproteins that are more important to the survival of the animal than is cG SHPx.
Journal ArticleDOI
Biochemical and molecular characterization of a barley seed beta-glucosidase.
TL;DR: The barley BGQ60 is a novel plant β-glucosidase with a hitherto undescribed specific enzymatic activity and the possible biological functions of BGZ60 during barley seed development and germination are discussed.
Journal ArticleDOI
Aspects of selenium metabolism in higher plants
TL;DR: The physiological differentiation within the genus Astragalus (one of the best known accumulators) is discussed, and the relationships between sulfur and selenium metabolism are discussed, along with the possible role of selenum as a micronutrient.
Journal ArticleDOI
Platelet glutathione peroxidase activity as an index of selenium status in rats.
TL;DR: Determination of glutathione peroxidase activity in platelets apparently is a promising technique for assessing selenium status and, possibly, for measuring selenum bioavailability.
Book ChapterDOI
[24] A general method for the preparation of plant mitochondria
TL;DR: This chapter describes a general method for the preparation of plant mitochondria, which combines control of temperature and pH with careful separation of the mitochondrial fraction from other materials that sediment with the mitochondria.
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