Journal ArticleDOI
Sensitive and specific detection of influenza virus A subtype H5 with real-time PCR.
J. Rossi,S. Cramer,T. Laue +2 more
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TLDR
A molecular test on the basis of real-time polymerase chain reaction (real-time PCR), which detects influenza virus RNA, enables highly sensitive detection of influenza virus A and B strains and specific identification of influenza A virus H5 subtypes.Abstract:
Avian influenza is a serious threat to both animal and human health. To enable cutting-edge research in this field, we developed a molecular test on the basis of real-time polymerase chain reaction (real-time PCR), which detects influenza virus RNA. The test enables highly sensitive detection of influenza virus A and B strains, including H5N1, and specific identification of influenza A virus H5 subtypes. The kit was tested against a broad panel of influenza A and B subtypes and other respiratory viruses in collaboration with worldwide authoritative laboratories and shows a very high specificity and sensitivity. An internal control verifies RNA extraction as well as real-time PCR success. With this kit, rapid and reliable detection of influenza A and B viruses and identification of H5 subtypes can be achieved.read more
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Journal ArticleDOI
Development and Validation of a One-Step Real-Time PCR Assay for Simultaneous Detection of Subtype H5, H7, and H9 Avian Influenza Viruses
Isabella Monne,Silvia Ormelli,Annalisa Salviato,Cristian De Battisti,Francesca Bettini,Angela Salomoni,A. Drago,Bianca Zecchin,Ilaria Capua,Giovanni Cattoli +9 more
TL;DR: This is the first real-time PCR protocol available for the simultaneous detection of AI viruses belonging to subtypes H5, H7, and H9, and the results obtained indicate that this method is suitable as a routine laboratory test for the rapid detection and differentiation of the three most-important AI virus subtypes in samples of avian origin.
Journal ArticleDOI
Development of Real Time RT-PCR Assays for Detection of Type A Influenza Virus and for Subtyping of Avian H5 and H7 Hemagglutinin Subtypes
Francesca Sidoti,Francesca Rizzo,Cristina Costa,Sara Astegiano,Antonio Curtoni,M. L. Mandola,Rossana Cavallo,Massimiliano Bergallo +7 more
TL;DR: Specific and sensitive real time reverse transcription PCR assays for the detection of type A influenza virus and for subtyping of avian H5 and H7 hemagglutinin subtypes are developed and compared with viral isolation in terms of sensitivity.
DissertationDOI
Sampling for airborne influenza virus using RNA preservation buffer : a new approach
TL;DR: In this article, the authors compare the efficacy of RNA preservation buffers (RNAPBs) over Hanks Balanced Salt Solution (HBSS) as a sample collection media and determine whether RNAPB stabilizes viral particles stored over time.
References
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Journal ArticleDOI
Genesis of a highly pathogenic and potentially pandemic H5N1 influenza virus in eastern Asia
K. S. Li,Yi Guan,Yi Guan,Jianbo Wang,Jianbo Wang,Gavin J. D. Smith,Gavin J. D. Smith,K. M. Xu,K. M. Xu,Lian Duan,Lian Duan,A. P. Rahardjo,Pilaipan Puthavathana,Chantanee Buranathai,Toan D. Nguyen,A. T. S. Estoepangestie,A. Chaisingh,Prasert Auewarakul,H. T. Long,N. T. H. Hanh,Richard J. Webby,Leo L.M. Poon,Honglin Chen,Honglin Chen,Kennedy F. Shortridge,Kennedy F. Shortridge,Kwok-Yung Yuen,Robert G. Webster,Robert G. Webster,Joseph S. M. Peiris,Joseph S. M. Peiris +30 more
TL;DR: The findings indicate that domestic ducks in southern China had a central role in the generation and maintenance of this virus, and that wild birds may have contributed to the increasingly wide spread of the virus in Asia.
Journal ArticleDOI
Application of real-time RT-PCR for the quantitation and competitive replication study of H5 and H7 subtype avian influenza virus.
Chang-Won Lee,David L. Suarez +1 more
TL;DR: A one-tube hydrolysis fluorescent probe based real-time RT-PCR based quantitation of AI virus, which is specific, sensitive, easy to perform, and rapid, will be useful for virological, pathogenesis, and protection studies.
Journal ArticleDOI
Pandemic influenza threat and preparedness.
TL;DR: New vaccine technologies and antiviral drugs are needed to prepare for the next influenza pandemic, according to the World Health Organization.
Journal ArticleDOI
Avian Flu to Human Influenza
TL;DR: Future vaccine strategies that may include more robust induction of T-cell responses, such as cytotoxic T lymphocytes, may provide better protection than is offered by current vaccines, which rely solely or mainly on antibody neutralization of infection.
Journal ArticleDOI
Development of a quantitative Light Cycler real-time RT-PCR for detection of avian reovirus.
Guan M. Ke,Hsueh L. Cheng,Liang Y. Ke,Wen T. Ji,Julius L.C. Chulu,Ming H. Liao,Tien J. Chang,Hung J. Liu +7 more
TL;DR: A robust, ultrasensitive, and accurate quantitative assay was developed for avian reovirus (ARV) with the Light Cycler SYBR Green-based real-time reverse transcription-PCR (real-time LC RT-PCRs), which exhibited high specificity as all negative controls and other avian pathogens failed to show any positive detection.