Sensitive capture of circulating tumour cells by functionalized graphene oxide nanosheets
Hyeun Joong Yoon,Tae Hyun Kim,Zhuo Zhang,Ebrahim Azizi,Trinh Pham,Costanza Paoletti,Jules Lin,Nithya Ramnath,Max S. Wicha,Daniel F. Hayes,Diane M. Simeone,Sunitha Nagrath +11 more
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TLDR
An effective approach to isolate CTCs from blood samples of pancreatic, breast and lung cancer patients is demonstrated by using functionalised graphene oxide nanosheets on a patterned gold surface.Abstract:
The spread of cancer throughout the body is driven by circulating tumour cells (CTCs). These cells detach from the primary tumour and move from the bloodstream to a new site of subsequent tumour growth. They also carry information about the primary tumour and have the potential to be valuable biomarkers for disease diagnosis and progression, and for the molecular characterization of certain biological properties of the tumour. However, the limited sensitivity and specificity of current methods for measuring and studying these cells in patient blood samples prevents the realization of their full clinical potential. The use of microfluidic devices is a promising method for isolating CTCs. However, the devices are reliant on three-dimensional structures, which limits further characterization and expansion of cells on the chip. Here we demonstrate an effective approach to isolating CTCs from blood samples of pancreatic, breast and lung cancer patients, by using functionalized graphene oxide nanosheets on a patterned gold surface. CTCs were captured with high sensitivity at a low concentration of target cells (73 ± 32.4% at 3-5 cells per ml blood).read more
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References
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Circulating Tumor Cells, Disease Progression, and Survival in Metastatic Breast Cancer
Massimo Cristofanilli,G. Thomas Budd,Matthew J. Ellis,Alison Stopeck,Jeri Matera,M. Craig Miller,James M. Reuben,Gerald V. Doyle,W. Jeffrey Allard,Leon W.M.M. Terstappen,Daniel F. Hayes +10 more
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Isolation of rare circulating tumour cells in cancer patients by microchip technology.
Sunitha Nagrath,Lecia V. Sequist,Shyamala Maheswaran,Daphne W. Bell,Daphne W. Bell,Daniel Irimia,Lindsey Ulkus,Matthew R. Smith,Eunice L. Kwak,Subba R. Digumarthy,Alona Muzikansky,Paula D. Ryan,Ulysses J. Balis,Ulysses J. Balis,Ronald G. Tompkins,Daniel A. Haber,Mehmet Toner +16 more
TL;DR: The CTC-chip successfully identified CTCs in the peripheral blood of patients with metastatic lung, prostate, pancreatic, breast and colon cancer in 115 of 116 samples, with a range of 5–1,281CTCs per ml and approximately 50% purity.
Journal ArticleDOI
Functionalized graphene sheets for polymer nanocomposites
T. Ramanathan,Ahmed Abdala,Ahmed Abdala,Sasha Stankovich,Dmitriy A. Dikin,Margarita Herrera-Alonso,Richard D. Piner,Richard D. Piner,Douglas H. Adamson,Hannes C. Schniepp,Xinqi Chen,Rodney S. Ruoff,Rodney S. Ruoff,SonBinh T. Nguyen,Ilhan A. Aksay,Robert K. Prud'homme,L. C. Brinson +16 more
TL;DR: Modulus, ultimate strength and thermal stability follow a similar trend, with values for functionalized graphene sheet- poly(methyl methacrylate) rivaling those for single-walled carbon nanotube-poly(methyl methamphetamine) composites.
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