Journal ArticleDOI
Separation of cell types in embryonic heart cell cultures
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TLDR
A technique is described for the separation of cell types in primary cell cultures which is dependent upon the different rates of attachment of each cell type to the substratum.About:
This article is published in Experimental Cell Research.The article was published on 1970-11-01. It has received 135 citations till now. The article focuses on the topics: Cell type & Cell culture.read more
Citations
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Immunoelectron microscopic studies of the sites of cell-substratum and cell-cell contacts in cultured fibroblasts.
W T Chen,S J Singer +1 more
TL;DR: A coherent but tentative scheme is proposed for the molecular ultrastructure of these contacts sites, and specific functional roles are suggested for fibronectin, vinculin, and alpha-actinin in cell adhesion and in the linkage of intracellular microfilaments to membranes at the different types of contact sites.
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Culturing of calcium stable adult cardiac myocytes
TL;DR: Cardiac myocytes isolated from adult rats can be kept in culture with physiological calcium concentrations up to four days, and over this time cardiocytes preserve their normal ultrastructure.
Journal ArticleDOI
Novel cell lines derived from adult human ventricular cardiomyocytes.
Mercy M. Davidson,Claudia Nesti,Lluis Palenzuela,Winsome F. Walker,Evelyn Hernandez,Lev Protas,Michio Hirano,Nithila D. Isaac +7 more
TL;DR: Based on the expression of myogenic markers and a fully functional respiratory chain, the AC cells have retained the nuclear DNA and the mitochondrial DNA of the primary cardiomyocytes and are potentially useful in vitro models to study developmental regulation of cardo-specific markers in normal and pathological states.
Journal ArticleDOI
Isolation and long-term cell culture of epithelial-like cells from rat liver.
TL;DR: Empithelial-like cells from livers of 10-day-old rats were separated from fibroblast- like cells on the basis of differential attachment to a culture surface and these maintained their morphology for up to nine months of continuous culture and displayed no tumorigenicity upon injection into syngeneic hosts.
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Elastic Fully Three-dimensional Microstructure Scaffolds for Cell Force Measurements
Franziska Klein,Thomas Striebel,Joachim E. Fischer,Zhongxiang Jiang,Clemens M. Franz,Georg von Freymann,Martin Wegener,Martin Bastmeyer +7 more
TL;DR: It is shown that biocompatible scaffolds can be fabricated by means of direct laser writing (DLW) and subsequent surface functionalization and it is demonstrated that these scaffolding can be rhythmically deformed by single beating cardiomyocytes.
References
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Journal ArticleDOI
A Requirement for Two Cell Types for Antibody Formation in vitro
TL;DR: It was found that both adherent and nonadherent cells were necessary for the induction of antibody formation to sheep red blood cells in vitro.
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Separation of lymphocytes, polymorphonuclear leukocytes and monocytes on glass columns, including tissue culture observations.
Yale Rabinowitz,Yale Rabinowitz +1 more
TL;DR: The viability of column-separated cells was shown by their non-staining with trypan blue, motility, phagocytic ability, oxygen consumption, and survival or development in tissue culture, whereas Nowell’s blast-like, dividing, phytohemagglutinin cells were produced only in cultures containing lymphocytes.
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Regulation of spontaneous activity and growth of embryonic chick heart cells in tissue culture
TL;DR: No necessary antagonism exists between growth and the maintenance of a differentiated functional state in embryonic heart cells cultured under the conditions described, and it is suggested that the high intracellular Na+-content and rapid Na-flux characteristic of early embryonic hearts represent devices whereby early pacemaker cells can maintain a ratio of sodium: potassium conductance sufficiently large to permit the onset of spontaneous activity in the high-potassium environment provided by the egg before amnion formation.
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Separation of various blood cells in colloidal silica-polyvinylpyrrolidone gradients
TL;DR: New methods are described for the separation of the cellular components of human blood by centrifugation in density gradients of colloidal silica and polyvinylpyrrolidone, with yields and resolution higher than in methods currently available.
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The effect of actinomycin d on heart and thigh muscle cells grown in vitro.
David Yaffe,Michael Feldman +1 more
TL;DR: It is suggested that the synthesis of proteins essential for the maintenance of the functional properties of muscle cells continues under conditions of suppression of DNA-dependent RNA synthesis.