Separation of progressive motile sperm from mouse semen using on-chip chemotaxis.
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TLDR
This device provides a convenient, disposable, and high-throughput platform that could function as a progressive motile sperm sorter for potential use in intracytoplasmic sperm injection.Abstract:
We present a novel method for the separation of progressive motile sperm from non-progressive motile and immotile sperm. This separation was accomplished by inducing chemotaxis along a longitudinal chemical gradient in a microchip composed of a biocompatible polydimethysiloxane layer and a glass substrate. In a preliminary experiment using fluorescent rhodamine B as a marker, we verified that a chemical gradient was generated by diffusion within the microchannel. We used acetylcholine as a chemoattractant to evaluate the chemotactic response of sperm. We tested the response to a 1/2 to 1/64 dilution series of acetylcholine. The results of a mouse sperm chemotaxis assay showed that progressive motile sperm swam predominantly toward the outlet at an optimal chemical gradient of 0.625 (mg/ml)/mm of acetylcholine. This device provides a convenient, disposable, and high-throughput platform that could function as a progressive motile sperm sorter for potential use in intracytoplasmic sperm injection.read more
Citations
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The influence of the female reproductive tract and sperm features on the design of microfluidic sperm-sorting devices
TL;DR: In this article , a review of FRT features, which can implement by microfluidics devices, focusing on sperm selection and investigation devices, was presented, where the authors tried not only point to the deficiencies of these systems, but also put forth suggestions for their improvement as well.
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Sperm Accumulation Induced by the Female Reproductive Fluid: Putative Evidence of Chemoattraction Using a New Tool.
TL;DR: In this paper, a 3D printed sperm selection chamber was used to measure sperm chemotaxis and in general chemoattraction in a zebrafish, which can be easily quantified by measuring sperm concentration.
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Investigating the factors affecting the outcomes of the sperm sorting with microfluidic devices
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N-formyl-l-aspartate: A novel sperm chemoattractant identified in ovulatory phase oviductal fluid using a microfluidic chip
TL;DR: In this article, sperm motility behavior in response to ovulatory (OV) and preovulatory (preOV) oviductal fluid (OF) was determined by spectrofluorimetry.
Book ChapterDOI
Microfluidic Devices for Gamete Processing and Analysis, Fertilization and Embryo Culture and Characterization
TL;DR: This chapter summarizes and discusses microfluidic developments in the field of ART, and specifically devices for gamete analysis, selection and processing, fertilization and embryo culture.
References
More filters
Journal ArticleDOI
Cells on chips
TL;DR: Highly integrated microdevices show great promise for basic biomedical and pharmaceutical research, and robust and portable point-of-care devices could be used in clinical settings, in both the developed and the developing world.
Journal ArticleDOI
Biotechnology at low Reynolds numbers
TL;DR: An intuitive explanation of how the different physics of small Reynolds numbers flow, along with microscopic sizes, can influence device design is presented, and examples from the own work using fluid flow in microfabricated devices designed for biological processing are given.
Journal ArticleDOI
Sperm guidance in mammals - an unpaved road to the egg.
TL;DR: The mechanisms by which mammalian sperm cells are guided to the egg are reviewed.
Journal ArticleDOI
A novel 3D mammalian cell perfusion-culture system in microfluidic channels
Yi-Chin Toh,Chi Zhang,Jing Zhang,Yuet Mei Khong,Shi Chang,Victor Samper,Danny van Noort,Dietmar W. Hutmacher,Hanry Yu +8 more
TL;DR: A transparent 3D microfluidic channel-based cell culture system that allows cells to be perfusion-cultured in 3D by supporting them with adequate 3D cell-cell and cell-matrix interactions and allows direct optical monitoring of cellular events for a wide range of applications.