Separation of progressive motile sperm from mouse semen using on-chip chemotaxis.
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TLDR
This device provides a convenient, disposable, and high-throughput platform that could function as a progressive motile sperm sorter for potential use in intracytoplasmic sperm injection.Abstract:
We present a novel method for the separation of progressive motile sperm from non-progressive motile and immotile sperm. This separation was accomplished by inducing chemotaxis along a longitudinal chemical gradient in a microchip composed of a biocompatible polydimethysiloxane layer and a glass substrate. In a preliminary experiment using fluorescent rhodamine B as a marker, we verified that a chemical gradient was generated by diffusion within the microchannel. We used acetylcholine as a chemoattractant to evaluate the chemotactic response of sperm. We tested the response to a 1/2 to 1/64 dilution series of acetylcholine. The results of a mouse sperm chemotaxis assay showed that progressive motile sperm swam predominantly toward the outlet at an optimal chemical gradient of 0.625 (mg/ml)/mm of acetylcholine. This device provides a convenient, disposable, and high-throughput platform that could function as a progressive motile sperm sorter for potential use in intracytoplasmic sperm injection.read more
Citations
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Journal ArticleDOI
Progesterone stimulates the long-distance migration of capacitated ram spermatozoa through viscous media under geotactic condition
C. Martínez-Rodríguez,L. Anel-López,Mercedes Alvarez,Cristina Ortega-Ferrusola,J.C. Boixo,Fernando J. Peña,Luis Anel,Paulino de Paz +7 more
TL;DR: An integrated analysis of long-distance ram sperm migration in vitro that combined two environmental factors (10 μM progesterone and a geotactic effect) and the physiological status of the cells (capacitation treatment) revealed migrating spermatozoa are morphologically better adapted for displacement in viscous media and exhibit remarkably depleted mitochondrial membrane potential.
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