scispace - formally typeset

Journal ArticleDOI

Sequence and secondary structure of Porphyra umbilicalis 5S rRNA. Relevance for the evolutionary origin of red algae

25 Nov 1988-Nucleic Acids Research (Oxford University Press)-Vol. 16, Iss: 22, pp 10919-10919

AboutThis article is published in Nucleic Acids Research.The article was published on 1988-11-25 and is currently open access. It has received 3 citation(s) till now. The article focuses on the topic(s): Porphyra umbilicalis & Red algae.

Topics: Porphyra umbilicalis (63%), Red algae (53%)

Summary (1 min read)

Jump to:  and [Summary]

Summary

  • This pattern can be explained by assuming that the ancestral, eukaryotic 5S rRNA shared the first two features with the archaebacterlal and eubacterial 5S rRNAs, and that these were altered in the eukaryotic branch of evolution only after the divergence of the red algae, the latter conserving these ancestral features until the present time.
  • As such, the study of 5S rRNA secondary structure consolidates phylogenetic studies (4) based on 5S rRNA sequences and pointing to the red algae as the earliest diverging branch of the eukaryotes.

Did you find this useful? Give us your feedback

...read more

Content maybe subject to copyright    Report

volume
16
Number
22 1988
Nucleic Acids Research
Sequence and secondary structure of Porphyra umbilicalis 5S rRNA. Relevance for the
evolutionary origin of red algae
Hilde Van den Eynde, Raymond De Baere and Rupert De Wachter
Departement Biochemie, Universiteit Antwerpen (UIA), Universiteitsplein 1, B-2610 Antwerp, Belgium
Submitted October 21, 1988 Accession no. X13222
The sequence
of the
nytoplasmic
5S
rRNA
of the red
alga Porphyra
umbilicalis
was
determined
and
fits
the
following secondary structure model:
A
M B I, C H,
C
AU
A
A
A
UG
C
C
CC
\
pACGUGCGGC GUGCGGAAC GCCGAAA
C
UGUACGCCG CACGCCUCG CGGCUUU
<J
U-G V
A
A
U
A-A
A
A
G
C°
GC
o
G
U
:
C
A
C
-G
2
A
U
G
A
G-C
U
GC
A
U
E C-G-Q
U'A'
C
-G
C
-G
H
2
A G
G
G
This model, applicable
to all red
algal
5S
rRNAs (1),
has
certain features
reminiscent
of
bacterial rather than eukaryotic
5S
rRNAs,
as
documented below:
feature
(see
Presence
of
N°
of
bases
Symmetry
of
Presence
of
also refs.
bulge
on
in helix
loop
I
bulge(s)
5'
C
on
2
and
-strand
+ loop
helix
3)
of
"l
E
Archae-
bacteria
helix
B yes
27
sym.
sometimes
Eubacteria
yes
27
sym.
no
Eukaryotes
red algae other
yes
27
asym.
yes
no
26
asym.
yes
This pattern
can be
explained
by
assuming that
the
ancestral, eukaryotic
5S
rRNA shared
the
first
two
features with
the
archaebacterlal
and
eubacterial
5S
rRNAs,
and
that these were altered
in the
eukaryotic branch
of
evolution only
after
the
divergence
of the red
algae,
the
latter conserving these ancestral
features until
the
present time.
As
such,
the
study
of 5S
rRNA secondary
structure consolidates phylogenetic studies
(4)
based
on 5S
rRNA sequences
and
pointing
to the red
algae
as the
earliest diverging branch
of the
eukaryotes.
References:
1) Lira,
B.-L. et al.
(1986)
Jpn. J.
Genet.
61,
169-176.
2) Willekens,
P. et al.
(1986) Syst. Appl. Microbiol.
7,
151-157.
3)
Van den
Eynde,
H., De
Wachter,
R.
(1987) FEBS Lett.
217,
191-196.
4) Hori,
H.,
Osawa,
S.
(1987)
Mol.
Biol. Evol.
4,
445-472.
© IRL
Press
Limited, Oxford, England. 10919
Citations
More filters

Book ChapterDOI
Sarah P. Gibbs1
01 Jan 1992
TL;DR: The present understanding of how chloroplasts evolved in the different groups of algae is summed up in this symposium presentation.
Abstract: The algae are not a natural assemblage of organisms Rather they are a diverse group of protists and fungi which have acquired chloroplasts in various ways, in some groups directly from symbiotic photosynthetic prokaryotes and in others from symbiotic eukaryotic algae The new techniques for rapidly sequencing ribosomal RNA, discussed by Adoutte in this volume, are producing evolutionary trees which are giving us a clearer understanding of the true relationships between different eukaryotes In Fig 1, I have drawn an evolutionary tree based on the sequences of the small subunit ribosomal RNA of a number of eukaryotes The most ancient eukaryotes whose ribosomal RNA has been sequenced to date are Giardia lamblia, a parasitic diplomonad, and Vairimorpha necatrix, a microsporidian Neither of these protists has mitochondria, and it is possible that these ancient eukaryotes evolved prior to the acquisition of mitochondria Also ancient are the trypanosomes and Euglena Somewhat later Dictyostelium evolved, but then came an explosive radiation of many protist groups, fungi, plants and animals In this tree, I have put in boldface lettering those groups which contain species with chloroplasts A glance at the tree shows that the algae are polyphyletic Euglenoids evolved very early and are related to trypanosomes The dinoflagellates, which many have assumed to be an ancient group because of the presence of a mesokaryotic nucleus (Loeblich, 1976), in fact evolved late and are related to ciliates The brown algae and Chrysophytes are related to each other and to the Oomycetes, a relationship long suspected due to the presence of heterokont flagella in each group Higher plants and green algae form a natural assemblage The fact that organisms with chloroplasts appear on different branches of the tree indicates that different groups have acquired chloroplasts independently of each other In this symposium presentation, I will summarize our present understanding of how chloroplasts evolved in the different groups of algae

68 citations


Journal ArticleDOI
Abstract: The Cyanidiophyceae members (PreRhodophyta) may serve as a transitional algal group bridging the cyanobacteria and the unicellular Rhodophyta. This thermoacidic algal group is composed of three genera containing several species. We suggested placing these algae in progressively evolutionary steps: (Cyanidioschyzon → Cyanidium → Galdieria). This evolutional ladder is based upon various areas of research like biochemistry, amount of nuclear genome and shape of chloroplast nucleoid, ultrastructure and ecological aspects. The first alga —Cyanidioschyzon — is the cornerstone of this succession; it shows mixed features between cyanobacterium and archaebacteria(Thermoplasma-like cell). It demonstrates simple eukaryotic cellular features and has the smallest amount of nuclear and chloroplast DNA. The intermediate alga in this line,Cyanidium, is also a simple cell, but shows more progressive characterizations than theCyanidioschyzon. The third taxon,Galdieria, is already very close to the unicellular rhodophytes (red algae) and indicates typical advanced eukaryotic characterization. We propose thatCyanidioschyzon (considered to be the simplest eukaryote) may have evolved from an association betweenThermoplasma-like archaebacterium and a thermophilic cyanobacterium. Autogenous (non-symbiotic) compartmental steps may have taken place fromCyanidioschyzon toCyanidium and then toGaldieria, and from this alga (group) towards the other unicellular red algae.

31 citations


Journal ArticleDOI
TL;DR: The myxobacterial 5S rRNA sequence data failed to confirm the existence of a delta subgroup of the class Proteobacteria, which was suggested by the results of 16S rRNAs, and a dendrogram was constructed by using weighted pairwise grouping based on these and all other previously known eubacterial 5 sRNA sequences.
Abstract: 5S rRNA sequences were determined for the myxobacteria Cystobacter fuscus, Myxococcus coralloides, Sorangium cellulosum, and Nannocystis exedens and for the radioresistant bacteria Deinococcus radiodurans and Deinococcus radiophilus. A dendrogram was constructed by using weighted pairwise grouping based on these and all other previously known eubacterial 5S rRNA sequences, and this dendrogram showed differences as well as similarities compared with results derived from 16S rRNA analyses. In the dendrogram, Deinococcus 5S rRNA sequences clustered with 5S rRNA sequences of the genus Thermus, as suggested by the results of 16S rRNA analyses. However, in contrast to the 16S rRNA results, the Deinococcus-Thermus cluster divided the 5S rRNA sequences of the alpha subdivision of the class Proteobacteria from the 5S rRNA sequences of the beta and gamma subgroups of the Proteobacteria. The myxobacterial 5S rRNA sequence data failed to confirm the existence of a delta subgroup of the class Proteobacteria, which was suggested by the results of 16S rRNA analyses.

9 citations


Frequently Asked Questions (1)
Q1. What contributions have the authors mentioned in the paper "Nucleic acids research sequence and secondary structure of porphyra umbilicalis 5s rrna. relevance for the evolutionary origin of red algae" ?

As such, the study of 5S rRNA secondary structure consolidates phylogenetic studies ( 4 ) based on 5S rRNA sequences and pointing to the red algae as the earliest diverging branch of the eukaryotes.