Sequencing technologies-the next generation
Summary (1 min read)
Summary
- DNA sequencing is one of the most important platforms for study in biological systems today.
- The high-throughput-next generation sequencing technologies delivers fast, inexpensive, and accurate genome information.
- Next generation sequencing can produce over 100 times more data than methods based on Sanger Sequencing.
- The next generation sequencing technologies offered from Illumina / Solexa, ABI/SOLiD, 454/Roche, and Helicos has provided unprecedented opportunity for high-throughput functional genomic research.
- Next generation sequence technologies offer novel and rapid ways for genome-wide characterization and profiling of mRNA's, transcription factor regions, and DNA patterns.
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Citations
245 citations
Cites background from "Sequencing technologies-the next ge..."
...As described in our previous study (67), DNA libraries based on the PCR products described above were constructed according to the manufacturer’s instructions (Illumina)....
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...In this study, the Illumina Solexa GA II method was used for a single read of 81 bp, and 11 species were separately sequenced....
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...A series of in-house Perl scripts was then employed for further quality control, and reads were culled according to the following criteria: (i) reads filtered with Illumina’s Consensus Assessment of Sequence and Variation (CASAVA) software, (ii) reads with no call sites, (iii) reads with similarity to the sequencing adaptor and the primer K sequence, and (iv) duplicate reads and low-complexity reads....
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...One bat virome analysis conducted by Ge et al. (18) with the Illumina Solexa GA method for a single read of 35 bp mainly described the insect viruses in some bat species of China....
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...The amplified viral nucleic acid libraries of the 11 bat species were then sequenced with an Illumina GA II (one lane per species; Table 1)....
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245 citations
Cites background from "Sequencing technologies-the next ge..."
...This development coupled with the high-throughput sequencing data produced by next-generation sequencing (NGS) technologies ensures an adequate depth of sequencing coverage to accurately detect the variants in the exome or targeted regions (Mamanova et al. 2010; Turner et al. 2010; Koboldt et al. 2010; Metzker 2010; Shendure and Ji 2008)....
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...…sequencing data produced by next-generation sequencing (NGS) technologies ensures an adequate depth of sequencing coverage to accurately detect the variants in the exome or targeted regions (Mamanova et al. 2010; Turner et al. 2010; Koboldt et al. 2010; Metzker 2010; Shendure and Ji 2008)....
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244 citations
242 citations
Cites methods from "Sequencing technologies-the next ge..."
...They allow for the sequencing of millions of short (few hundreds bp) DNA fragments (reads) simultaneously and may process a whole human genome in three days at 500-fold less cost than previous methods (Voelkerding et al., 2009; Metzker, 2010)....
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241 citations
Cites background from "Sequencing technologies-the next ge..."
...In addition, the new sequencing capabilities driven by rapid technological advances will find applications within barley breeding (including MAS) as it has already in genomic research (Metzker 2009; Varshney et al. 2009)....
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References
11,528 citations
"Sequencing technologies-the next ge..." refers background in this paper
...For example, in gene-expression studies microarrays are now being replaced by seq-based methods , which can identify and quantify rare transcripts without prior knowledge of a particular gene and can provide information regarding alternative splicing and sequence variation in identified gene...
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9,389 citations
8,434 citations
4,490 citations
"Sequencing technologies-the next ge..." refers background in this paper
...and to elucidate the role of non-coding RNAs in health and diseas...
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4,277 citations
"Sequencing technologies-the next ge..." refers background in this paper
...and to elucidate the role of non-coding RNAs in health and diseas...
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