Sequencing technologies-the next generation
Summary (1 min read)
Summary
- DNA sequencing is one of the most important platforms for study in biological systems today.
- The high-throughput-next generation sequencing technologies delivers fast, inexpensive, and accurate genome information.
- Next generation sequencing can produce over 100 times more data than methods based on Sanger Sequencing.
- The next generation sequencing technologies offered from Illumina / Solexa, ABI/SOLiD, 454/Roche, and Helicos has provided unprecedented opportunity for high-throughput functional genomic research.
- Next generation sequence technologies offer novel and rapid ways for genome-wide characterization and profiling of mRNA's, transcription factor regions, and DNA patterns.
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Citations
150 citations
Cites background from "Sequencing technologies-the next ge..."
...Next generation sequencing technologies are generating massive sequence data [1], and different platforms can introduce varied level of sequence reads error....
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150 citations
150 citations
150 citations
149 citations
Cites background or methods from "Sequencing technologies-the next ge..."
...NGS benefits greatly to patients with rare cancers and cancer of unknown primary site, for detailed genomic profiling could be used to identify the main drivers of malignant transformation and to cover the shortage of diagnosis and treatment strategies [294, 295]....
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...CAGE Cap analysis of gene expression CID Collision induced dissociation CML Chronic myelogenous leukemia ECD Electro capture dissociation ETD Electron transfer dissociation FTICR Fourier transform ion cyclotron resonance GC Gas chromatography HCC Hepatocellular carcinoma HP Fucosylated haptoglobin HPLC High performance liquid chromatography IARC International Agency for Research on Cancer ICGC International Cancer Genome Consortium lncRNAs Long ncRNAs LC Liquid chromatography LSCC Lung squamous cell carcinoma MALDI Matrix-assisted laser desorption ionization MDLC Multi-dimensional LC MPSS Massively parallel signature sequencing MRM Multiple reaction monitoring MS Mass spectrometry MS/MS Tandem mass spectrometry NANA N-acetylneuraminic acid ncRNAs Non-coding RNAs NGS Next-generation sequencing NMR Nuclear magnetic resonance NSCLC Non-small cell lung cancer PPPM Predictive, preventive, and personalized medicine SAGE Serial analysis of gene expression SCLC Small cell lung cancer SCO Small cell osteosarcoma * Xianquan Zhan yjzhan2011@gmail.com 1 Key Laboratory of Cancer Proteomics of ChineseMinistry of Health, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, Hunan 410008, People’s Republic of China 2 Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, Hunan 410008, People’s Republic of China 3 State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, Hunan 410008, People’s Republic of China 4 The State Key Laboratory of Medical Genetics, Central South University, 88 Xiangya Road, Changsha, Hunan 410008, People’s Republic of China SRM Selected reaction monitoring SWATH Sequential window acquisition of all theoretical mass spectra TCGA Cancer Genome Atlas TOF Time-of-flight 1DGE One-dimensional gel electrophoresis 2DGE Two-dimensional gel electrophoresis 2DDIGE Two-dimensional difference in-gel electrophoresis WGS Whole genome sequencing...
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...The disadvantage that limited the application of these platforms are shorter read lengths with an average read length range from 32 to 330 bp [37]), which creates challenges for genome alignment and assemble [3, 37, 38, 40, 41]....
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...999% [38], Sanger sequencing achieved a number of monumental accomplishments, including completing of the Human Genome Project [37]....
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...The second-and third-generation technologies are referred to as NGS [37]....
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References
11,528 citations
"Sequencing technologies-the next ge..." refers background in this paper
...For example, in gene-expression studies microarrays are now being replaced by seq-based methods , which can identify and quantify rare transcripts without prior knowledge of a particular gene and can provide information regarding alternative splicing and sequence variation in identified gene...
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"Sequencing technologies-the next ge..." refers background in this paper
...and to elucidate the role of non-coding RNAs in health and diseas...
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4,277 citations
"Sequencing technologies-the next ge..." refers background in this paper
...and to elucidate the role of non-coding RNAs in health and diseas...
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