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Open AccessJournal ArticleDOI

Sero-epidemiological survey for alveolar echinococcosis (by Em2-ELISA) of blood donors in an endemic area of Switzerland

Bruno Gottstein, +7 more
- 01 Nov 1987 - 
- Vol. 81, Iss: 6, pp 960-964
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TLDR
Sera from 17,166 blood donors living in 10 cantons of northern Switzerland in an area endemic for Echinococcus multilocularis were investigated by serological survey for alveolar echinococcosis, finding 2 asymptomatic clinical cases of AE within this population of blood donors.
Abstract: 
Sera from 17 166 blood donors living in 10 cantons of northern Switzerland in an area endemic for Echinococcus multilocularis were investigated by serological survey for alveolar echinococcosis (AE). A highly species-specific antigen (Em2) and a commonly used E. granulosus hydatid fluid antigen (EgHF) were compared for their suitability in seroepidemiology. EgHF showed a degree of nonspecificity which did not allow direct detection of AE cases. Antibody reaction with Em2 resulted in the detection of 2 asymptomatic clinical cases of AE (seroprevalence 0·01%) within this population of blood donors. A further 4 persons were positive in Em2-ELISA. These 4 persons had negative imaging studies and will be followed serologically and clinically.

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Citations
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Journal ArticleDOI

Dot immunogold filtration assay (DIGFA) with multiple native antigens for rapid serodiagnosis of human cystic and alveolar echinococcosis.

Xiaohui Feng, +9 more
- 01 Feb 2010 - 
TL;DR: A new 3-min rapid dot immunogold filtration assay for serodiagnosis of human cystic and alveolar echinococcosis was developed and can be used for both clinical diagnostic support, as well as in conjunction with ultrasound for mass screening in endemic CE and AE areas.
Book ChapterDOI

Echinococcus multilocularis Infection: Immunology and Immunodiagnosis

Bruno Gottstein
- 01 Jan 1992 - 
TL;DR: This chapter presents the immunological aspect of E. multilocularis and also discusses various diagnostic techniques and new developments in the diagnosis of and vaccination against E. multi-ocularis are discussed.
Journal ArticleDOI

The immunodiagnosis of Echinococcus multilocularis infection.

David Carmena, +2 more
- 01 May 2007 - 
TL;DR: An overview of the present situation regarding the immunodiagnosis of E. multilocularis infection is presented, particularly the detection of excretory-secretory and integument products of the worm in faeces (copro-antigens) by ELISA.
Journal ArticleDOI

Application of a recombinant Echinococcus multilocularis antigen in an enzyme-linked immunosorbent assay for immunodiagnosis of human alveolar echinococcosis.

Norbert Müller, +4 more
- 01 Sep 1989 - 
TL;DR: Investigation of sera from 220 patients with various helminthic infections showed a specificity of 99%, suggesting the suitability of the antigen II/3-10 as an immunodiagnostic tool.
Journal ArticleDOI

Sequencing and characterization of an Echinococcus multilocularis DNA probe and its use in the polymerase chain reaction.

Bruno Gottstein, +1 more
- 01 Feb 1991 - 
TL;DR: The nucleotide sequence of the cloned Echinococcus multilocularis DNA probe pAL1 was determined in order to simplify and improve the sensitivity of a diagnostic assay through the application of the polymerase chain reaction (PCR).
References
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Journal ArticleDOI

Chemotherapy for larval echinococcosis in animals and humans: report of a workshop

Peter M. Schantz, +2 more
- 01 Jan 1982 - 
TL;DR: Treatment of human patients infected with E. granulosus is followed by subjective improvement in most, and evidence of regression of cysts in some; in other patients, cysts continue to grow or have been proven viable even after several months of high-dose mebendazole therapy.
Journal ArticleDOI

Spontaneous death of Echinococcus multilocularis: cases diagnosed serologically (by Em2 ELISA) and clinical significance.

Robert L. Rausch, +3 more
- 01 May 1987 - 
TL;DR: The findings show that the Em2 ELISA may be positive in patients having lesions of AHD in which the etiologic agent is no longer viable, in agreement with the clinical impression that the cestode was dead.
Journal ArticleDOI

Serological differentiation between Echinococcus granulosus and E. multilocularis infections in man.

Bruno Gottstein, +2 more
- 01 Jan 1983 - 
TL;DR: An enzyme-linked immunosorbent assay (ELISA) was adapted for the serological differential diagnosis of cystic or alveolar echinococcosis in man caused by Echinococcus granulosus or E. multilocularis respectively.
Journal ArticleDOI

Purification and characterization of a specific antigen from Echinococcus multilocularis.

Bruno Gottstein
- 01 May 1985 - 
TL;DR: A polypeptide (Em2a) purified by affinity chromatography from the Echinococcus multilocularis metacestode showed a high degree of purity as assayed by SDS‐PAGE and analytical isoelectrical focusing and a minor contamination with host albumin was revealed.
Journal ArticleDOI

Determination of parasite-specific immunoglobulins using the ELISA in patients with echinococcosis treated with mebendazole

Bruno Gottstein, +2 more
- 01 Jan 1984 - 
TL;DR: Parasite-specific immunoglobulin classes were determined by the ELISA in sera of 19 patients with cystic or alveolar echinococcosis before and after 2 years of continuous treatment with mebendazole at daily doses of 35–50 mg/kg body weight.
Related Papers (5)

Spontaneous death of Echinococcus multilocularis: cases diagnosed serologically (by Em2 ELISA) and clinical significance.

Robert L. Rausch, +3 more
- 01 May 1987 - 

Serological differentiation between Echinococcus granulosus and E. multilocularis infections in man.

Bruno Gottstein, +2 more
- 01 Jan 1983 - 

Seroepidemiologic screening of Echinococcus multilocularis infection in a European area endemic for alveolar echinococcosis

Solange Bresson-Hadni, +9 more
- 01 Dec 1994 - 

Improved primary immunodiagnosis of alveolar echinococcosis in humans by an enzyme-linked immunosorbent assay using the Em2plus antigen.

Bruno Gottstein, +3 more
- 01 Feb 1993 - 

Purification and characterization of a specific antigen from Echinococcus multilocularis.

Bruno Gottstein
- 01 May 1985 - 
Frequently Asked Questions (1)
Q1. What are the contributions in "Sero-epidemiological survey for alveolar echinococcosis (by em2-elisa) of blood donors in an endemic area of switzerland" ?

Gottstein et al. this paper compared a highly species-specific antigen ( Em2 ) and a commonly used E. granulosus hydatid fluid antigen ( EgHF ) for their suitability in seroepidemiology.