Serum enzymes as indicators of chemically induced liver damage.
TL;DR: Serum ornithine carbamyl transferase (SOCT) activity increased at least 6-fold in animals that showed liver damage by histopathology, and fell again as the injuries resolved, while Measurements of other enzymes were less sensitive.
Abstract: Rats were dosed with CCl4 or diethylamine to induce liver injury. The time and magnitude of peak liver injury were assessed by histopathological examination of liver specimens taken at intervals after dosing. Serum enzymes were measured at the same intervals. Serum ornithine carbamyl transferase (SOCT) activity increased at least 6-fold in animals that showed liver damage by histopathology, and fell again as the injuries resolved. Measurements of other enzymes were less sensitive. SOCT measurements appear to be as sensitive a method as histopathology for detecting liver damage caused by administering xenobiotics. Since serum enzyme measurements do not require that the animals be sacrificed, they can be used for repeated examinations of the same animals, thus increasing the likelihood of detecting transient injury.
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TL;DR: The results of this study suggest that SNE could protect liver against the CCl4-induced oxidative damage in rats, and this hepatoprotective effect might be contributed to its modulation on detoxification enzymes and its antioxidant and free radical scavenger effects.
222 citations
TL;DR: Here, a dose-dependent study was conducted and oral administration of Kamilari at a dose of 750 mg/kg body weight significantly reduced the toxic effects of CCl4.
183 citations
TL;DR: Zanthoxylum armatum DC possesses significant protective effect against hepatotoxicity induced by CCl(4) which may be attributed to the individual or combined action of phytoconstituents present in it.
162 citations
Journal Article•
146 citations
TL;DR: It was observed that Cytisus scoparius extract protects liver from oxidative stress induced by CCl(4) in rats and thus helps in evaluation of the traditional claim on this plant.
139 citations
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Book•
01 Jan 1963
TL;DR: Methods of enzymatic analysis, Methods of enzymes analysis, the authors, Methods of enzyme analysis, enzymatics, methods of enzymes, and methods of analysis, method of enzymes.
Abstract: Methods of enzymatic analysis , Methods of enzymatic analysis , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی
18,100 citations
TL;DR: Methods for determination of isocitric and 6-phosphogluconic dehydrogenases in human blood serum are described and the properties of these enzymes, and factors affecting their activity in the blood sera of normal human adults are discussed.
Abstract: SummaryMethods for determination of isocitric and 6-phosphogluconic dehydrogenases in human blood serum are described. The properties of these enzymes, and factors affecting their activity in the blood sera of normal human adults are discussed.
136 citations
TL;DR: Sorbol dehydrogenase and fructose-1-phosphate aldolase appear to hold promise as indicators of minimal liver damage and dose dependent over a wide dose range.
95 citations
TL;DR: Minimal morphological changes were seen in liver sections at dosage levels of each compound below those required to produce changes in serum enzyme activity, and sorbitol dehydrogenase was the most sensitive serum parameter studied.
64 citations
TL;DR: Serum enzyme levels and isozyme profile were utilized as a measure of hepatotoxic response to carbon tetrachloride, mercuric chloride, diethanolamine and thioacetamide, finding that advanced degenerative change, including necrosis, had occurred in both the liver and kidney before enzyme alterations were seen.
40 citations