SH-SY5Y human neuroblastoma cell line: in vitro cell model of dopaminergic neurons in Parkinson's disease.
Hongrong Xie,Lin-sen Hu,Guoyi Li +2 more
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TLDR
Evaluating the human neuroblastoma SH-SY5Y cell line as an in vitro model of dopaminergic (DAergic) neurons for Parkinson's disease (PD) research and the effect of differentiation on this cell model found some differentiating agents afford SH- SY5Y cells with more potential for studying neurotoxicity and neuroprotection and are thus more relevant to experimental PD research.Abstract:
ObjectiveTo evaluate the human neuroblastoma SH-SY5Y cell line as an in vitro model of dopaminergic (DAergic) neurons for Parkinson's disease (PD) research and to determine the effect of differentiation on this cell model.Data sourcesThe data of this review were selected from the original reports anread more
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Book ChapterDOI
Considerations for the use of SH-SY5Y neuroblastoma cells in neurobiology.
Jane Kovalevich,Dianne Langford +1 more
TL;DR: The popular SH-SY5Y neuroblastoma cell line and its use in in vitro systems is described and Retinoic acid is the most commonly used means for differentiation and will be addressed in detail.
Journal ArticleDOI
The SH-SY5Y cell line in Parkinson's disease research: a systematic review
TL;DR: An overview of the cell source, culture conditions, differentiation protocols, methods/approaches used to mimic PD and the preclinical validation of the SH-SY5Y findings by employing alternative cellular and animal models is described.
Journal ArticleDOI
Complexity of dopamine metabolism
TL;DR: This review highlights different aspects of dopamine metabolism in the context of PD and neurodegeneration by looking at DA biosynthesis, sequestration, degradation and oxidation chemistry at the metabolic level, as well as at the transcriptional, translational and posttranslational regulation of all enzymes involved.
Journal ArticleDOI
Engineered in vitro disease models.
Kambez H. Benam,Stephanie Dauth,Bryan Hassell,Anna Herland,Abhishek Jain,Kyung-Jin Jang,Katia Karalis,Katia Karalis,Hyun-Jung Kim,Luke A. MacQueen,Roza Mahmoodian,Samira Musah,Yu Suke Torisawa,Andries D. van der Meer,Remi Villenave,Moran Yadid,Kevin Kit Parker,Donald E. Ingber +17 more
TL;DR: E engineered in vitro models of diseases of the heart, lung, intestine, liver, kidney, cartilage, skin and vascular, endocrine, musculoskeletal, and nervous systems, as well as models of infectious diseases and cancer are provided.
Journal ArticleDOI
Assessing bioenergetic function in response to oxidative stress by metabolic profiling
Brian P. Dranka,Gloria A. Benavides,Anne R. Diers,Samantha Giordano,Blake R. Zelickson,Colin Reily,Luyun Zou,John C. Chatham,Bradford G. Hill,Jianhua Zhang,Aimee Landar,Victor M. Darley-Usmar +11 more
TL;DR: Methods for assessing the impact of reactive species on isolated mitochondria are described using representative cell types from renal, cardiovascular, nervous, and tumorigenic model systems while illustrating the application of three protocols to analyze the bioenergetic response of cells to oxidative stress.
References
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Neurotrophin signal transduction in the nervous system.
David R. Kaplan,Freda D. Miller +1 more
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Morphology and Growth, Tumorigenicity, and Cytogenetics of Human Neuroblastoma Cells in Continuous Culture
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Role of Polyamines in the Control of Cell Proliferation and Differentiation
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Sequential Treatment of SH‐SY5Y Cells with Retinoic Acid and Brain‐Derived Neurotrophic Factor Gives Rise to Fully Differentiated, Neurotrophic Factor‐Dependent, Human Neuron‐Like Cells
Mario Encinas,Montse Iglesias,Yuhui Liu,Hongyin Wang,Ashraf Muhaisen,Valentín Ceña,Carme Gallego,Joan X. Comella +7 more
TL;DR: This model may be useful to perform large‐scale biochemical and molecular studies due to its susceptibility to genetic manipulation and the availability of an unlimited amount of cells.
Journal ArticleDOI
Retinoic acid-induced differentiation of cultured human neuroblastoma cells: a comparison with phorbolester-induced differentiation.
TL;DR: The results suggest that RA and TPA induce the SH-SY5Y cells to differentiate along different pathways, with an increased neuron-specific enol enzyme activity, relative to the total cellular enolase activity.
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Considerations for the use of SH-SY5Y neuroblastoma cells in neurobiology.
Jane Kovalevich,Dianne Langford +1 more