Silk scaffolds with tunable mechanical capability for cell differentiation
TL;DR: These silk fibroin scaffolds regulate cell differentiation outcomes due to their mechanical features, which resulted in different differentiation outcomes with rat bone marrow-derived mesenchymal stem cells toward myogenic and endothelial cells, respectively.
About: This article is published in Acta Biomaterialia.The article was published on 2015-07-01 and is currently open access. It has received 80 citations till now. The article focuses on the topics: Fibroin & SILK.
Citations
More filters
TL;DR: A type of bioinspired bone adhesive is able to provide stable fracture fixation and accelerated bone regeneration during the bone remodeling process and promotes the regeneration of bone defects at an early stage in vivo.
109 citations
TL;DR: The mechanistic basis for the entrapment and stabilization features, along with insights into the modulation of release of the entrained compounds from silks will be reviewed with a focus on stabilization of bioactive molecules.
106 citations
TL;DR: A review of recent approaches for guiding MSC fate using biomaterials is highlighted and a description of the underlying characteristics that promote differentiation toward a desired phenotype is provided.
Abstract: Cell-based therapies are a promising alternative to grafts and organ transplantation for treating tissue loss or damage due to trauma, malfunction, or disease. Over the past two decades, mesenchymal stem cells (MSCs) have attracted much attention as a potential cell population for use in regenerative medicine. Although the proliferative capacity and multilineage potential of MSCs provide an opportunity to generate clinically relevant numbers of transplantable cells, their use in tissue regenerative applications has met with relatively limited success to date apart from secreting paracrine-acting factors to modulate the defect microenvironment. Presently, there is significant effort to engineer the biophysical properties of biomaterials to direct MSC differentiation and further expand on the potential of MSCs in tissue engineering, regeneration, and repair. Biomaterials can dictate MSC differentiation by modulating features of the substrate including composition, mechanical properties, porosity, and topogr...
92 citations
TL;DR: In this paper, Lvqiang78@suda.edu.cn et al. proposed a model for textile and clothing engineering at Soochow University in China, which is based on the work of the Kaplan National Engineering Laboratory for Modern Silk & Collaborative Innovation Center of Suzhou Nano Science and Technology.
Abstract: Prof. Q. Lu, Dr. S. Bai, Prof. D. L. Kaplan National Engineering Laboratory for Modern Silk & Collaborative Innovation Center of Suzhou Nano Science and Technology Soochow University Suzhou 215123 , P. R. China E-mail: Lvqiang78@suda.edu.cn Prof. Q. Lu, Dr. S. Bai, Prof. D. L. Kaplan College of Textile and Clothing Engineering Soochow University Suzhou 215123 , P. R. China Z. Ding School of Biology and Basic Medical Sciences Soochow University Suzhou 215123 , P. R. China Dr. H. Guo, Prof. Z. Shao Key Laboratory of Molecular Engineering of Polymers of Ministry of Education Laboratory of Advanced Materials Department of Macromolecular Science Fudan University Shanghai 200433 , P. R. China Prof. H. Zhu Research Center of Materials Science Beijing Institute of Technology Beijing 100081 , P. R. China Prof. D. L. Kaplan Department of Biomedical Engineering Tufts University Medford , MA 02155 , USA
83 citations
TL;DR: In vitro and in vivo osteogenesis studies demonstrated that the composite scaffolds showed improved osteogenesis capacity under suitable BMP-2 release conditions, significantly better than that of B MP-2 loaded SF-HA composite scaffolding reported previously.
Abstract: Osteoinductive biomaterials are attractive for repairing a variety of bone defects, and biomimetic strategies are useful toward developing bone scaffolds with such capacity. Here, a multiple biomimetic design was developed to improve the osteogenesis capacity of composite scaffolds consisting of hydroxyapatite nanoparticles (HA) and silk fibroin (SF). SF nanofibers and water-dispersible HA nanoparticles were blended to prepare the nanoscaled composite scaffolds with a uniform distribution of HA with a high HA content (40%), imitating the extracellular matrix (ECM) of bone. Bone morphogenetic protein-2 (BMP-2) was loaded in the SF scaffolds and HA to tune BMP-2 release. In vitro studies showed the preservation of BMP-2 bioactivity in the composite scaffolds, and programmable sustained release was achieved through adjusting the ratio of BMP-2 loaded on SF and HA. In vitro and in vivo osteogenesis studies demonstrated that the composite scaffolds showed improved osteogenesis capacity under suitable BMP-2 rel...
79 citations
References
More filters
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
12,204 citations
TL;DR: Studies with well-defined silkworm silk fibers and films suggest that the core silk fibroin fibers exhibit comparable biocompatibility in vitro and in vivo with other commonly used biomaterials such as polylactic acid and collagen.
3,067 citations
TL;DR: These distinctive in vivo 3D-matrix adhesions differ in structure, localization, and function from classically described in vitro adhesion, and as such they may be more biologically relevant to living organisms.
Abstract: Adhesions between fibroblastic cells and extracellular matrix have been studied extensively in vitro, but little is known about their in vivo counterparts. Here, we characterized the composition and function of adhesions in three-dimensional (3D) matrices derived from tissues or cell culture. "3D-matrix adhesions" differ from focal and fibrillar adhesions characterized on 2D substrates in their content of alpha5beta1 and alphavbeta3 integrins, paxillin, other cytoskeletal components, and tyrosine phosphorylation of focal adhesion kinase (FAK). Relative to 2D substrates, 3D-matrix interactions also display enhanced cell biological activities and narrowed integrin usage. These distinctive in vivo 3D-matrix adhesions differ in structure, localization, and function from classically described in vitro adhesions, and as such they may be more biologically relevant to living organisms.
3,000 citations
TL;DR: The persistence of the engrafted hMSCs and their in situ differentiation in the heart may represent the basis for using these adult stem cells for cellular cardiomyoplasty.
Abstract: Background— Cellular cardiomyoplasty has been proposed as an alternative strategy for augmenting the function of diseased myocardium. We investigated the potential of human mesenchymal stem cells (hMSCs) from adult bone marrow to undergo myogenic differentiation once transplanted into the adult murine myocardium. Methods and Results— A small bone marrow aspirate was taken from the iliac crest of healthy human volunteers, and hMSCs were isolated as previously described. The stem cells, labeled with lacZ, were injected into the left ventricle of CB17 SCID/beige adult mice. At 4 days after injection, none of the engrafted hMSCs expressed myogenic markers. A limited number of cells survived past 1 week and over time morphologically resembled the surrounding host cardiomyocytes. Immunohistochemistry revealed de novo expression of desmin, β-myosin heavy chain, α-actinin, cardiac troponin T, and phospholamban at levels comparable to those of the host cardiomyocytes; sarcomeric organization of the contractile pro...
2,317 citations
TL;DR: This protocol includes methods to extract silk from B. mori cocoons to fabricate hydrogels, tubes, sponges, composites, fibers, microspheres and thin films, used directly as biomaterials for implants, as scaffolding in tissue engineering and in vitro disease models, as well as for drug delivery.
Abstract: Silk fibroin, derived from Bombyx mori cocoons, is a widely used and studied protein polymer for biomaterial applications. Silk fibroin has remarkable mechanical properties when formed into different materials, demonstrates biocompatibility, has controllable degradation rates from hours to years and can be chemically modified to alter surface properties or to immobilize growth factors. A variety of aqueous or organic solvent-processing methods can be used to generate silk biomaterials for a range of applications. In this protocol, we include methods to extract silk from B. mori cocoons to fabricate hydrogels, tubes, sponges, composites, fibers, microspheres and thin films. These materials can be used directly as biomaterials for implants, as scaffolding in tissue engineering and in vitro disease models, as well as for drug delivery.
2,165 citations